糖尿病大鼠主动脉中RhoA易位增加。

Increased RhoA translocation in aorta of diabetic rats.

作者信息

Tang Jiping, Kusaka Ikuyo, Massey Amber R, Rollins Shadon, Zhang John H

机构信息

Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, MS 39216, USA.

出版信息

Acta Pharmacol Sin. 2006 May;27(5):543-8. doi: 10.1111/j.1745-7254.2006.00325.x.

DOI:10.1111/j.1745-7254.2006.00325.x

PMID:16626508

Abstract

AIM

To analyze RhoA expression and activation in the aorta of diabetic rats.

METHODS

Male SD rats (n=70) were divided into 2 groups: the diabetic group and the control group. Diabetes was induced by intravenous injection of streptozotocin (55 mg/kg). The Rats were studied 3 weeks after the induction of diabetes. Western blotting was used to measure the expression and activation of Rho.

RESULTS

Heart rate was measured 24 h/d; it decreased by 58+/-13 beats/min in the diabetic rats. Isometric tension showed that the contraction of diabetic aorta was significantly reduced compared with that of control aorta when stimulated by KCl and serotonin. The relaxation of the diabetic aorta was reduced when stimulated by acetylcholine. An enhanced RhoA translocation in the aortic tissues of diabetic rats was determined by a 90% increase in membrane-bound RhoA, indicating that the activation of RhoA is markedly increased in the diabetic aorta.

CONCLUSION

Our data suggest that upregulated RhoA could be involved in the vascular dysfunction of diabetic rats.

摘要

目的

分析糖尿病大鼠主动脉中RhoA的表达及激活情况。

方法

将70只雄性SD大鼠分为2组：糖尿病组和对照组。通过静脉注射链脲佐菌素（55 mg/kg）诱导糖尿病。在诱导糖尿病3周后对大鼠进行研究。采用蛋白质印迹法检测Rho的表达及激活情况。

结果

每天24小时测量心率；糖尿病大鼠心率降低了58±13次/分钟。等长张力显示，当用氯化钾和5-羟色胺刺激时，糖尿病大鼠主动脉的收缩与对照主动脉相比显著降低。当用乙酰胆碱刺激时，糖尿病大鼠主动脉的舒张减弱。通过膜结合RhoA增加90%确定糖尿病大鼠主动脉组织中RhoA转位增强，表明糖尿病大鼠主动脉中RhoA的激活明显增加。