Huang Dong, Ge Jun-bo, Liang Chun, Luo Yu-kun, Jia Qing-zhe
Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Zhonghua Xin Xue Guan Bing Za Zhi. 2006 Feb;34(2):148-52.
To study the changes of lectin-like oxidized low density lipoprotein receptor-1 (LOX-1) expression in the autologous vein grafts and vein graft atherosclerotic lesions.
Thirty New Zealand white rabbits were randomly assigned to normal control group (rabbits fed with normal diet, n = 10), vein graft group (autologous external jugular vein grafting to common carotid artery and fed with normal diet, n = 10) or vein graft plus high-lipid diet group (autologous vein graft and fed with high-lipid diet, n = 10) for 12 weeks. LOX-1 expressions in the grafts were examined by immunohistochemistry and semi-quantitative reverse transcription-PCR. The relationships between serum total cholesterol level, intimal thickness and LOX-1 expression were also investigated.
LOX-1 expression was low in the endothelium of external jugular veins in the normal control group and significantly increased in the endothelium and neointima of vein grafts in the vein graft group (0.31 +/- 0.14 vs. 0.09 +/- 0.04, P < 0.01) and which was further increased in the endothelium and atherosclerotic lesions in the vein graft plus high-lipid diet group (0.93 +/- 0.34 vs. 0.31 +/- 0.14, P < 0.01). LOX-1 expression in the atherosclerotic lesions was located both in endothelial cells and foam cells and the expression was most prominent in endothelial cells. LOX-1 expression and intimal thickness were positively related to serum total cholesterol level (P = 0.00 and 0.02) and the partial correlation coefficient was 0.78 and 0.42, respectively.
LOX-1 expression is increased in endothelium and neointima of autologous vein grafts of rabbits. Hypercholesterolemia upregulates LOX-1 expression in vein graft atherosclerosis. Thus, LOX-1 might play an important role in the pathogenesis of vein graft atherosclerosis.
研究凝集素样氧化低密度脂蛋白受体-1（LOX-1）在自体静脉移植物及静脉移植物动脉粥样硬化病变中的表达变化。
将30只新西兰白兔随机分为正常对照组（喂正常饮食的兔子，n = 10）、静脉移植物组（自体颈外静脉移植至颈总动脉并喂正常饮食，n = 10）或静脉移植物加高脂饮食组（自体静脉移植物并喂高脂饮食，n = 10），为期12周。通过免疫组织化学和半定量逆转录聚合酶链反应检测移植物中LOX-1的表达。还研究了血清总胆固醇水平、内膜厚度与LOX-1表达之间的关系。
正常对照组颈外静脉内皮中LOX-1表达较低，静脉移植物组静脉移植物的内皮和新生内膜中LOX-1表达显著增加（0.31±0.14对0.09±0.04，P < 0.01），而静脉移植物加高脂饮食组的内皮和动脉粥样硬化病变中LOX-1表达进一步增加（0.93±0.34对0.31±0.14，P < 0.01）。动脉粥样硬化病变中LOX-1表达位于内皮细胞和泡沫细胞中，且在内皮细胞中表达最为突出。LOX-1表达和内膜厚度与血清总胆固醇水平呈正相关（P = 0.00和0.02），偏相关系数分别为0.78和0.42。
兔自体静脉移植物的内皮和新生内膜中LOX-1表达增加。高胆固醇血症上调静脉移植物动脉粥样硬化中LOX-1的表达。因此，LOX-1可能在静脉移植物动脉粥样硬化的发病机制中起重要作用。
