尿皮质素2和尿皮质素3在人胎盘、蜕膜和胎膜中表达。
Urocortin 2 and urocortin 3 are expressed by the human placenta, deciduas, and fetal membranes.
作者信息
Imperatore Alberto, Florio Pasquale, Torres Paulo B, Torricelli Michela, Galleri Letizia, Toti Paolo, Occhini Rossella, Picciolini Enrico, Vale Wylie, Petraglia Felice
机构信息
Department of Pediatrics, Obstetrics and Reproductive Medicine, Section of Obstetrics and Gynecology, University of Siena, Siena, Italy.
出版信息
Am J Obstet Gynecol. 2006 Jul;195(1):288-95. doi: 10.1016/j.ajog.2005.12.048. Epub 2006 Apr 19.
OBJECTIVE
Urocortin 2 (UCN2) and urocortin 3 (UCN 3) are recently identified neuropeptides showing homology to corticotropin-releasing factor (CRF). In the present study, we evaluated their expression and localization in gestational tissues (placenta, decidua, fetal membranes), and their effect on placental adrenocorticotropic hormone secretion.
STUDY DESIGN
The study was performed in a tertiary clinical care center. Tissues were obtained at first (n = 8; 8-11 weeks of pregnancy) and third (n = 8; 38-40 gestational weeks) trimester. The mRNA expression was evaluated by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR); the cellular localization by immunohistochemistry; ACTH levels were measured in media collected from cultured placental villi.
RESULTS
All tissues analyzed expressed UCN2 and UCN3 mRNA. UCN2 and UCN3 were localized in cytotrophoblast and syncytiotrophoblast cells; UCN2 was present in maternal and fetal vessels and in amniotic cells, while UCN3 was absent. Finally, UCN2 and UCN3 did not stimulate ACTH secretion.
CONCLUSION
Gestational tissues differentially express UCN2 and UCN3 and, despite their homology to CRF, they did not stimulate placental ACTH secretion.
目的
尿皮质素2（UCN2）和尿皮质素3（UCN3）是最近发现的与促肾上腺皮质激素释放因子（CRF）具有同源性的神经肽。在本研究中，我们评估了它们在妊娠组织（胎盘、蜕膜、胎膜）中的表达和定位，以及它们对胎盘促肾上腺皮质激素分泌的影响。
研究设计
本研究在一家三级临床护理中心进行。在孕早期（n = 8；妊娠8 - 11周）和孕晚期（n = 8；妊娠38 - 40周）获取组织。通过半定量逆转录 - 聚合酶链反应（RT-PCR）评估mRNA表达；通过免疫组织化学确定细胞定位；在从培养的胎盘绒毛收集的培养基中测量促肾上腺皮质激素（ACTH）水平。
结果
所有分析的组织均表达UCN2和UCN3 mRNA。UCN2和UCN3定位于细胞滋养层和合体滋养层细胞；UCN2存在于母血和胎儿血管以及羊膜细胞中，而UCN3不存在。最后，UCN2和UCN3未刺激ACTH分泌。
结论
妊娠组织差异表达UCN2和UCN3，尽管它们与CRF具有同源性，但它们并未刺激胎盘ACTH分泌。
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