Hagedorn M, Carter V L, Steyn R A, Krupp D, Leong J C, Lang R P, Tiersch T R
Department of Reproductive Sciences, Smithsonian's National Zoological Park, Washington, DC 20008, USA.
Cryobiology. 2006 Jun;52(3):454-8. doi: 10.1016/j.cryobiol.2006.03.001.
Coral species throughout the world are facing severe environmental pressures. Because of this, we began cryobiological studies on the sperm of the mushroom coral, Fungia scutaria. We determined that F. scutaria sperm had a mean length of 56 microm and head diameter of 2.5 microm, and a mean spontaneous ice nucleation temperature of -37.2 +/- 1.7 degrees C. When the sperm were exposed to the cryoprotectant glycerol for 5 or 20 min (at 10% v/v), no fertilized larvae were produced. However, when sperm were exposed for 20 min to propylene glycol (10% v/v), fertilizations were produced at the same rate as untreated control eggs and sperm (P > 0.05), but slightly less for dimethyl sulfoxide (10% v/v) (P < 0.05). Regardless, dimethyl sulfoxide caused less osmotic damage to the sperm membrane than did propylene glycol. Therefore, we used the dimethyl sulfoxide (10% v/v) to develop cryopreservation protocols that yielded good post-thaw morphology and motility (>95%) for coral sperm.
世界各地的珊瑚物种都面临着严峻的环境压力。因此，我们开始对盾形陀螺珊瑚（Fungia scutaria）的精子进行低温生物学研究。我们测定出盾形陀螺珊瑚精子的平均长度为56微米，头部直径为2.5微米，平均自发冰核形成温度为-37.2±1.7摄氏度。当精子在10%（v/v）的冷冻保护剂甘油中暴露5或20分钟后，未产生受精幼虫。然而，当精子在10%（v/v）的丙二醇中暴露20分钟时，受精率与未处理的对照卵和精子相同（P>0.05），但在10%（v/v）的二甲基亚砜中受精率略低（P<0.05）。无论如何，二甲基亚砜对精子膜造成的渗透损伤比丙二醇小。因此，我们使用10%（v/v）的二甲基亚砜制定了冷冻保存方案，该方案能使珊瑚精子在解冻后具有良好的形态和活力（>95%）。
