Ohsawa Yutaka, Murakami Tatsufumi, Miyazaki Yuko, Shirabe Teruo, Sunada Yoshihide
Division of Neurology, Department of Internal Medicine, Kawasaki Medical School, 577 Matsushima, Kurashiki City, Okayama, Japan.
J Neurol Sci. 2006 Aug 15;247(1):11-5. doi: 10.1016/j.jns.2006.03.004. Epub 2006 Apr 19.
We studied the expression of peripheral myelin protein 22 (PMP22) gene in the human central nervous system (CNS). Northern blot analysis was performed with polyA+ RNA blots containing several parts of the human brain and the spinal cord using human PMP22 cDNA as a probe. As two alternative PMP22 transcripts have been reported and since exon 1A-containing transcripts are associated with myelin formation, the exon 1A fragment was also used to examine this transcript. Total PMP22 mRNA was significantly detected in most parts of brain and spinal cord, while exon 1A-containing transcripts were detected in the medulla, spinal cord and corpus callosum. PMP22-like immunoreactivity was identified in motor neurons and preganglionic sympathetic neurons in the spinal cord. PMP22 was also detected in pia mater of the spinal cord. These results suggest that PMP22 might play an important role in human CNS.
我们研究了外周髓鞘蛋白22（PMP22）基因在人类中枢神经系统（CNS）中的表达。使用人PMP22 cDNA作为探针，对包含人脑和脊髓多个部分的聚腺苷酸加尾RNA印迹进行Northern印迹分析。由于已报道了两种可变的PMP22转录本，且含外显子1A的转录本与髓鞘形成相关，因此外显子1A片段也用于检测该转录本。在脑和脊髓的大部分区域均显著检测到总PMP22 mRNA，而在延髓、脊髓和胼胝体中检测到含外显子1A的转录本。在脊髓的运动神经元和节前交感神经元中鉴定出PMP22样免疫反应性。在脊髓软膜中也检测到了PMP22。这些结果表明，PMP22可能在人类中枢神经系统中发挥重要作用。
