Helms Mike W, Brandt Burkhard H, Contag Christopher H
Institute of Pathology, University of Muenster, Muenster, Germany.
Contrib Microbiol. 2006;13:209-231. doi: 10.1159/000092975.
Detection and observation of primary tumor growth and metastasis in living subjects is an important task in clinical and basic cancer research. Recently several approaches and techniques emerged which offer a huge variety of options with respect to the specific objectives and questions of a given study. Recent developments in the field of in vivo imaging not only allow the assessment of anatomic information but also functional processes with cellular resolution and molecular sensitivity. This chapter will provide an overview of the most common imaging techniques which are currently available for the detection and observation of metastasizing tumor cells. General capacities, advantages, limitations and drawbacks will be discussed. These techniques include computed tomography (CT), molecular resonance imaging (MRI), positron emission tomography (PET), single photon emission computed tomography (SPECT), fluorescence imaging (FI), and bioluminescent imaging (BLI). The objective is to provide the cancer researcher with information that will help solve the dilemma of how best to apply the latest imaging tools for studying biological questions in the context of the living body.
在活体受试者中检测和观察原发性肿瘤的生长和转移是临床和基础癌症研究中的一项重要任务。最近出现了几种方法和技术，针对特定研究的具体目标和问题提供了多种多样的选择。体内成像领域的最新进展不仅能够评估解剖学信息，还能以细胞分辨率和分子灵敏度评估功能过程。本章将概述目前可用于检测和观察转移性肿瘤细胞的最常见成像技术。将讨论这些技术的一般能力、优点、局限性和缺点。这些技术包括计算机断层扫描（CT）、分子共振成像（MRI）、正电子发射断层扫描（PET）、单光子发射计算机断层扫描（SPECT）、荧光成像（FI）和生物发光成像（BLI）。目的是为癌症研究人员提供信息，以帮助解决如何最好地应用最新成像工具在活体环境中研究生物学问题的困境。
