Nguyen DiemThuy T, Bui Linda T, Ambrose Peter J
Long Beach Memorial Medical Center, Long Beach, California 90801-1428, USA.
Ther Drug Monit. 2006 Apr;28(2):252-4. doi: 10.1097/01.ftd.0000194028.89235.8d.
Bitter orange has recently been substituted as an ingredient in many "ephedra-free" dietary supplements used for weight loss. The primary active ingredient in bitter orange is synephrine. Previous reports have documented false-positive results from ephedrine with urine amphetamine assays. Because of the similarity in chemical structure of ephedrine and synephrine, it is hypothesized that ingestion of a bitter orange supplement may have the potential to cause false-positive results with urine amphetamine assays. The purpose of this study was to determine the response of the CEDIA Amphetamines Assay after ingestion of bitter orange. Six healthy adult male volunteers were administered a single oral dose of Nature's Way Bitter Orange, a 900-mg dietary supplement extract standardized to 6% synephrine. Urine specimens were collected at baseline and 3 and 6 hours post-administration. Additional urine specimens were collected from 1 subject at 9, 12, and 15 hours after administration. All specimens were analyzed by the CEDIA Amphetamines Assay. Urine specific gravity and pH also were measured. All urine specimens demonstrated a negative response to the CEDIA Amphetamines Assay. Urine specific gravity ranged from 1.007 to 1.028, and pH ranged from 5.0 to 7.0; thus, reducing the possibility that the negative results were caused by diluted specimens or reduced excretion of synephrine into alkaline urine. This information will be of value when health care providers or those who interpret drug screens are asked to provide consultation regarding the interference of bitter orange supplements with the CEDIA Amphetamines Assay. A single-dose of Nature's Way Bitter Orange was not found to cause a false-positive response to the CEDIA Amphetamines Assay in 6 healthy adult male volunteers.
苦橙最近已被用作许多用于减肥的“无麻黄碱”膳食补充剂的成分。苦橙中的主要活性成分是辛弗林。先前的报告记录了尿液苯丙胺检测中麻黄碱出现的假阳性结果。由于麻黄碱和辛弗林在化学结构上相似，因此推测摄入苦橙补充剂可能会导致尿液苯丙胺检测出现假阳性结果。本研究的目的是确定摄入苦橙后CEDIA苯丙胺检测的反应。六名健康成年男性志愿者单次口服一剂自然之宝苦橙，这是一种900毫克的膳食补充剂提取物，标准化为含6%辛弗林。在基线以及给药后3小时和6小时收集尿液样本。在给药后9小时、12小时和15小时从一名受试者收集额外的尿液样本。所有样本均通过CEDIA苯丙胺检测进行分析。还测量了尿比重和pH值。所有尿液样本对CEDIA苯丙胺检测均呈阴性反应。尿比重范围为1.007至1.028，pH值范围为5.0至7.0；因此，降低了阴性结果是由稀释样本或辛弗林排泄到碱性尿液中减少所致的可能性。当医疗保健提供者或解读药物筛查结果的人员被要求就苦橙补充剂对CEDIA苯丙胺检测的干扰提供咨询时，这些信息将很有价值。在六名健康成年男性志愿者中，未发现单剂量的自然之宝苦橙会导致CEDIA苯丙胺检测出现假阳性反应。
