一种用于非天然多肽合成的高度灵活的tRNA酰化方法。
A highly flexible tRNA acylation method for non-natural polypeptide synthesis.
作者信息
Murakami Hiroshi, Ohta Atsushi, Ashigai Hiroshi, Suga Hiroaki
机构信息
Research Center for Advanced Science and Technology, The University of Tokyo, 153-8904 Tokyo, Japan.
出版信息
Nat Methods. 2006 May;3(5):357-9. doi: 10.1038/nmeth877.
DOI:10.1038/nmeth877
PMID:16628205
Abstract
Here we describe a de novo tRNA acylation system, the flexizyme (Fx) system, for the preparation of acyl tRNAs with nearly unlimited selection of amino and hydroxy acids and tRNAs. The combination of the Fx system with an appropriate cell-free translation system allows us to readily perform mRNA-encoded synthesis of proteins and short polypeptides involving multiple non-natural amino acids.
摘要
在此，我们描述了一种用于制备酰基化tRNA的全新tRNA酰化系统——柔性酶（Fx）系统，该系统对氨基酸、羟基酸和tRNA的选择几乎不受限制。Fx系统与合适的无细胞翻译系统相结合，使我们能够轻松地进行涉及多种非天然氨基酸的mRNA编码蛋白质和短多肽的合成。
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