Yu Hai-Ling, An Ying, Bing Yan-Hua, Jin Qing-Hua, Cui Xun, Jin Yuan-Zhe
Department of Pharmacology, College of Medicine, Yanbian University, Yanji 133000, China.
Sheng Li Xue Bao. 2006 Apr 25;58(2):177-82.
In order to understand whether some special amino acids in the medial vestibular nucleus (MVN) of rats are involved in the regulation of blood pressure, we used microdialysis technique and high performance liquid chromatography (HPLC) to measure the changes of glutamate and taurine in this central area. Acute hypotension was induced by hemorrhage from the femoral artery. It was observed that the basal release of glutamate and taurine in the MVN was stable about 90 min after the beginning of microdialysis. The basal release of glutamate was (18.96 +/- 0.27) pmol/sample (8 mul), and that of taurine was (7.73 +/- 0.05) pmol/sample (8 mul). Glutamate release increased (P<0.05) and taurine release reduced (P<0.05) in the MVN in the hemorrhage-induced acute hypotensive rats. Nevertheless, these changes were not observed in the hemorrhage-induced acute hypotensive rats which were pretreated by infusing 2% lidocaine into the middle ear or 100 mg arsanilic acid into the tympanic cavity. These results suggest that the hemorrhage-induced acute hypotention can influence the activity of the neurons in the MVN by the afferent impulses from vestibular organ, and that some special amino acid transmitters in the MVN are involved in this process.
为了了解大鼠内侧前庭核（MVN）中的某些特殊氨基酸是否参与血压调节，我们采用微透析技术和高效液相色谱法（HPLC）来测定该中枢区域谷氨酸和牛磺酸的变化。通过股动脉放血诱导急性低血压。观察到微透析开始约90分钟后，MVN中谷氨酸和牛磺酸的基础释放稳定。谷氨酸的基础释放量为（18.96±0.27）pmol/样本（8μl），牛磺酸的基础释放量为（7.73±0.05）pmol/样本（8μl）。在出血诱导的急性低血压大鼠中，MVN中的谷氨酸释放增加（P<0.05），牛磺酸释放减少（P<0.05）。然而，在通过向中耳注入2%利多卡因或向鼓腔注入100mg对氨基苯胂酸预处理的出血诱导急性低血压大鼠中未观察到这些变化。这些结果表明，出血诱导的急性低血压可通过前庭器官的传入冲动影响MVN中神经元的活动，并且MVN中的某些特殊氨基酸递质参与了这一过程。
