Wu Hui-Juan, Li Xiao-Xin, Dong Jian-Qiang, Pei Wei-Hua, Chen Hong-Da
Department of Ophthalmology, People's Hospital, Peking University, Peking, People's Republic of China.
Graefes Arch Clin Exp Ophthalmol. 2007 Jan;245(1):135-42. doi: 10.1007/s00417-006-0296-4. Epub 2006 Apr 21.
Subretinal microphotodiode array (MPDA) is a type of visual prosthesis used for the implantation in the subretinal space of patients with progressive photoreceptor cell loss. The present study aimed to evaluate the effect of materials for MPDA on the viability, apoptosis and barrier function of cultured pig retinal pigment epithelium (RPE) cells.
Primary culture of pig RPE cells was performed and 24 pig eyes were used to start RPE culture. The third passage of the cultures was plated on different materials for MPDA and MPDAs. The tetrazolium dye-reduction assay (MTT) was used to determine RPE cell viability. Flow cytometry was measured to indicate the apoptosis rates of RPE cells on different materials. RPE cells were also cultured on microporous filters, and the transepithelial resistance and permeability of the experimental molecule were measured to determine the barrier function.
The data from all the methods indicated no significant difference between the materials groups and the control group, and the materials tested showed good biocompatibility.
The materials for MPDA used in the present study had no direct toxicity to the RPE cells and did not release harmful soluble factors that affected the barrier function of RPE in vitro.
视网膜下微光电二极管阵列（MPDA）是一种视觉假体，用于植入患有进行性光感受器细胞丧失的患者的视网膜下间隙。本研究旨在评估MPDA材料对培养的猪视网膜色素上皮（RPE）细胞的活力、凋亡和屏障功能的影响。
进行猪RPE细胞的原代培养，使用24只猪眼开始RPE培养。将第三代培养物接种到用于MPDA和MPDAs的不同材料上。采用四唑盐还原试验（MTT）测定RPE细胞活力。通过流式细胞术测量不同材料上RPE细胞的凋亡率。还将RPE细胞培养在微孔滤膜上，测量实验分子的跨上皮电阻和通透性以确定屏障功能。
所有方法的数据均表明材料组与对照组之间无显著差异，所测试的材料显示出良好的生物相容性。
本研究中使用的MPDA材料对RPE细胞无直接毒性，且未释放影响体外RPE屏障功能的有害可溶性因子。
