Nagasawa Masayuki, Isoda Takeshi, Itoh Sukeyuki, Kajiwara Michiko, Morio Tomohiro, Shimizu Norio, Ogawa Kazuyuki, Nagata Kinya, Nakamura Masataka, Mizutani Shuki
Department of Pediatrics and Developmental Biology, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
Am J Hematol. 2006 May;81(5):340-8. doi: 10.1002/ajh.20570.
Granulysin is a newly identified CTL/NK cell-related cytotoxic protein, which is secreted in both constitutive and Ca-dependent manner. To evaluate its significance in stem-cell transplantation (SCT), serum granulysin was measured by newly established ELISA method in 26 patients undergoing SCT (21 allogeneic and 5 autologous). In the allogeneic SCT, granulysin was transiently increased in 3 weeks, which was not observed in autologous SCT. In acute GVHD, serum granulysin was markedly increased and correlated with the severity of GVHD. Elevation of granulysin was not necessarily associated with increase of sIL2R or IFN-gamma. In vitro, allospecific T cells released granulysin in an allo-specific manner, and it was correlated with allo-specific cytotoxic activity. These results indicate that increased release of granulysin presents alloreactivity and serum granulysin is useful as a marker of GVHD in SCT.
颗粒溶素是一种新发现的与细胞毒性T淋巴细胞/自然杀伤细胞相关的细胞毒性蛋白，其分泌方式既有组成型分泌又有钙依赖性分泌。为评估其在干细胞移植（SCT）中的意义，采用新建立的酶联免疫吸附测定（ELISA）法检测了26例接受SCT患者（21例同种异体移植和5例自体移植）的血清颗粒溶素水平。在同种异体SCT中，颗粒溶素在3周内短暂升高，自体SCT中未观察到这种情况。在急性移植物抗宿主病（GVHD）中，血清颗粒溶素显著升高，且与GVHD的严重程度相关。颗粒溶素升高不一定与可溶性白细胞介素2受体（sIL2R）或γ干扰素（IFN-γ）升高相关。在体外，同种异体特异性T细胞以同种异体特异性方式释放颗粒溶素，且与同种异体特异性细胞毒性活性相关。这些结果表明，颗粒溶素释放增加提示同种异体反应性，血清颗粒溶素可作为SCT中GVHD的一个标志物。
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