Furudate Sadanori, Fujimura Taku, Kambayashi Yumi, Aiba Setsuya
Department of Dermatology, Tohoku University Graduate School of Medicine, Sendai, Japan.
Case Rep Dermatol. 2013 Apr 5;5(1):93-8. doi: 10.1159/000350561. Print 2013 Jan.
Graft-versus-host disease (GVHD) is an important complication of bone marrow transplantation and is known to induce sweat gland abnormalities. We employed immunohistochemical staining for granulysin, dermcidin as well as IL-17 and Foxp3 in the lesional skin of 7 patients with acute GVHD. Granulysin-bearing cells were distributed in the epidermis, basal membrane zone of the dermis, and superficial perivascular lesion of the dermis. Interestingly, granulysin-bearing cells were also detected around the secretory portion of the eccrine glands, which might be related to the downregulation of the expression of dermcidin on the secretory portion of the eccrine glands. Differing from a mouse model, in human acute GVHD, IL-17-producing cells and Foxp3(+) cells were not prominent. Our present observation sheds light on the contribution of granulysin-bearing cells to the decrease of the sweat in patients with acute GVHD. Though we did not assess the function of these infiltrating lymphocytes directly, further analysis of the mechanism of this phenomenon would offer fundamental insight into the establishment of acute GVHD.
移植物抗宿主病（GVHD）是骨髓移植的一种重要并发症，已知会导致汗腺异常。我们对7例急性移植物抗宿主病患者的皮损进行了颗粒溶素、皮肤杀菌肽以及白细胞介素-17和叉头框蛋白3的免疫组化染色。含颗粒溶素的细胞分布于表皮、真皮基底膜带及真皮浅层血管周围病变处。有趣的是，在小汗腺分泌部周围也检测到了含颗粒溶素的细胞，这可能与小汗腺分泌部皮肤杀菌肽表达下调有关。与小鼠模型不同，在人类急性移植物抗宿主病中，产生白细胞介素-17的细胞和叉头框蛋白3阳性细胞并不突出。我们目前的观察结果揭示了含颗粒溶素的细胞对急性移植物抗宿主病患者出汗减少的作用。虽然我们没有直接评估这些浸润淋巴细胞的功能，但对这一现象机制的进一步分析将为急性移植物抗宿主病的发病机制提供重要见解。
