Lönnberg Tuomas
Department of Chemistry, University of Turku, Vatselankatu 2, FIN-20014 Turku, Finland.
Nucleosides Nucleotides Nucleic Acids. 2006 Mar;25(3):315-23. doi: 10.1080/15257770500544537.
A phosphate-branched oligonucleotide has been prepared by using an appropriately protected trinucleoside phosphotriester building block in conventional solid-phase synthesis. Hydrolysis of the branched oligonucleotide has been followed over a wide pH range. Comparison of the present results with those previously obtained for simpler analogues indicates that a trinucleoside 3',3',5'-monophosphate, when embedded in an oligonucleotide structure, is stabilized toward hydroxide-ion catalyzed cleavage by more than one order of magnitude, lending some support to the feasibility of existence of phosphate-branched RNA X in biological systems.
通过在传统固相合成中使用适当保护的三核苷磷酸三酯结构单元制备了一种磷酸分支寡核苷酸。已在很宽的pH范围内跟踪了分支寡核苷酸的水解情况。将目前的结果与先前针对更简单类似物获得的结果进行比较表明，当嵌入寡核苷酸结构中时，三核苷3',3',5'-单磷酸对氢氧根离子催化的裂解稳定了一个多数量级，这为生物系统中磷酸分支RNA X存在的可行性提供了一些支持。
