Arredondo Miguel, Martínez Ronny, Núñez Marco T, Ruz Manuel, Olivares Manuel
Micronutrients Laboratory, Institute of Nutrition and Food Technology (INTA), Universidad de Chile.
Biol Res. 2006;39(1):95-102. doi: 10.4067/s0716-97602006000100011.
Interactions of micronutrients can affect absorption and bioavailability of other nutrients by a number of mechanisms. In aqueous solutions, and at higher uptake levels, competition between elements with similar chemical characteristics and uptake process can take place. The consequences of these interactions may depend on the relative concentrations of the nutrients. In this work, we measure the effects of increasing concentrations of iron, zinc, and copper on iron and copper uptake in Caco-2 cells. Intracellular Fe or Cu levels were affected by incubating with increased concentrations of metals. However, when the cells already had different intracellular metal concentration, the uptake of Fe or Cu was nor affected. In competition studies, we showed that Cu and Zn inhibited Fe uptake, and while Fe inhibited Cu uptake, Zn did not. When the three metals were given together (1:1:1 ratio), Fe or Cu uptake was inhibited approximately 40%. These results point to a potential risk in the absorption and bioavailability of these minerals by the presence of other minerals in the diet. This aspect must be considered in food supplementation and fortification programs.
微量营养素之间的相互作用可通过多种机制影响其他营养素的吸收和生物利用度。在水溶液中以及较高摄取水平下，具有相似化学特性和摄取过程的元素之间会发生竞争。这些相互作用的后果可能取决于营养素的相对浓度。在这项研究中，我们测量了铁、锌和铜浓度增加对Caco-2细胞中铁和铜摄取的影响。通过用浓度增加的金属进行孵育，细胞内铁或铜的水平受到了影响。然而，当细胞已经具有不同的细胞内金属浓度时，铁或铜的摄取并未受到影响。在竞争研究中，我们发现铜和锌抑制铁的摄取，虽然铁抑制铜的摄取，但锌没有。当三种金属一起给予（1:1:1比例）时，铁或铜的摄取被抑制了约40%。这些结果表明，饮食中其他矿物质的存在可能会对这些矿物质的吸收和生物利用度产生潜在风险。在食品补充和强化计划中必须考虑这一方面。
