Nihonyanagi Shin, Hirata Yasuyosi, Akabosi Tooru, Uchiyama Yukinobu, Yamaura Noboru, Sunakawa Keisuke, Inoue Matsuhisa
Department of Medical Laboratory, Kitasato University Hospital.
Kansenshogaku Zasshi. 2006 Mar;80(2):97-102. doi: 10.11150/kansenshogakuzasshi1970.80.97.
Isolated of multidrug resistance Pseudomonas aeruginosa (MDRP) that the receptivity pattern of the antimicrobial suscepti respectively resembled isolated from clinical specimens (sputum) in two patients of each internal medicine ward in Kitasato University East Hospital for two days from September 18 and 20, 2004. Both of bacteria were formed small colonies of a smooth-type on dollargalluskey improvement-type BTB agar plates, and the judgment of ClassB (metallo)-beta-lactamase by biochemical properties and disk diffusion method sodium mercaoto-acetic acid (SMA) was mutually corresponding. Moreover, it was same serotype C according to the serotype, and it was confirmed that it was the same bacterial strain from the molecular epidemiology analysis by Random amplified polymorphic DNA polymerase chain reaction (Random amplified polymorphic DNA polymerase chain reaction: RAPD). From the investigation of clinical backgrounds of two patients who isolated bacterial strains, September 18, 2004. 10 : 20 a.m., and 10 : 40 a.m., other chances that can become with contact infection in this hospital, except conducted X-Ray or roentgenograph of the chest and abdomen of Portable X-ray device continuously done by one radiation technician was not seen. Because it had turned out that a radiation technician who had taken charge had been neglecting the hand washing at the time of each X-Ray or roentgenograph, it was guessed the case with nosocomial infection by contact infection occurred via specific radiation technician.
2004年9月18日和20日两天，从北里大学东医院各内科病房的两名患者的临床标本（痰液）中分离出多重耐药铜绿假单胞菌（MDRP），其抗菌药敏模式分别相似。两种细菌在多尔加卢斯基改良型BTB琼脂平板上均形成光滑型小菌落，通过生化特性和纸片扩散法对巯基乙酸钠（SMA）进行B类（金属）-β-内酰胺酶的判断相互对应。此外，根据血清型为同一血清型C，通过随机扩增多态性DNA聚合酶链反应（随机扩增多态性DNA聚合酶链反应：RAPD）进行分子流行病学分析，确认是同一菌株。从分离出菌株的两名患者的临床背景调查来看，2004年9月18日上午10:20和上午10:40，除了由一名放射技师连续进行便携式X射线设备的胸部和腹部X射线或透视检查外，未见该医院内可能发生接触感染的其他情况。由于已查明负责的放射技师在每次X射线或透视检查时都疏于洗手，推测是通过特定放射技师发生接触感染导致的医院感染病例。
