Dauncey M J, Burton K A, Morovat A
Department of Molecular and Cellular Physiology, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge.
Exp Physiol. 1991 Nov;76(6):967-70. doi: 10.1113/expphysiol.1991.sp003558.
The maximal ouabain binding capacity (Bmax) of longissimus dorsi muscle has been determined over a 24 h period in young pigs living at 12 degrees C. Animals were fed once a day and Bmax was estimated at 4, 8, 12 or 24 h after a large meal. Mean values of Bmax +/- S.E.M. (pmol ouabain/g wet wt muscle) of 548 +/- 78 and 556 +/- 57 at 4 and 24 h after feeding were significantly greater than those of 383 +/- 24 and 393 +/- 30 at 8 and 12 h after feeding (P less than 0.02). The extent to which these differences represent apparent or real changes in numbers of ouabain binding sites and, hence, in Na+,K(+)-ATPase concentration is discussed.
已在12摄氏度环境下饲养的幼猪中，于24小时内测定了背最长肌的最大哇巴因结合能力（Bmax）。动物每天喂食一次，在一顿大餐后的4、8、12或24小时估计Bmax。喂食后4小时和24小时的Bmax±标准误（每克湿重肌肉中哇巴因的皮摩尔数）平均值分别为548±78和556±57，显著高于喂食后8小时和12小时的383±24和393±30（P<0.02）。讨论了这些差异在多大程度上代表哇巴因结合位点数量的表观或真实变化，进而代表钠钾ATP酶浓度的变化。
