Kim C-S, Sohn S-H, Jeon S-K, Kim K-N, Ryu J-J, Kim M-K
Department of Dentistry, College of Medicine, Korea University, Seoul, South Korea.
J Oral Rehabil. 2006 May;33(5):368-79. doi: 10.1111/j.1365-2842.2005.01553.x.
During the process of bone formation, titanium (Ti) surface is an important factor in the modulation of osteoblastic function. This study was conducted in order to determine the effects of different Ti surfaces on the biological responses of a human osteoblast-like cell line (MG63). MG63 cells were cultured on smooth (S), sandblasted large-grit and acid etching (SLA), hydroxyapatite (HA), hydroxyfluoride (HF), titanium nitrate (TIN), and diamond-like carbon (DLC) Ti. The morphology of these cells were assessed by SEM. The cDNAs prepared from the total RNAs of the MG63 were hybridized into a human cDNA microarray (1152 elements). The appearances of the surfaces observed by SEM were different on each of the six dental substrate types. The SLA and HA surfaces were determined to be rougher than the others. MG63 cells cultured on SLA and HA exhibited cell-matrix interactions. In the expression of genes involved in osseointegration, several genes, including bone morphogenetic protein, cadherin, integrin, and insulin-like growth factors, were upregulated on the different surfaces. Several genes, including fibroblast growth factor receptor 4, Bcl 2-related protein, and collagen, were downregulated on the different surfaces. The attachment and expression of key osteogenic regulatory genes were enhanced by the surface roughness of the dental materials used.
在骨形成过程中，钛（Ti）表面是调节成骨细胞功能的一个重要因素。进行本研究是为了确定不同钛表面对人成骨样细胞系（MG63）生物学反应的影响。MG63细胞在光滑（S）、喷砂大颗粒及酸蚀（SLA）、羟基磷灰石（HA）、氢氟酸（HF）、硝酸钛（TIN）和类金刚石碳（DLC）钛表面上培养。通过扫描电子显微镜（SEM）评估这些细胞的形态。从MG63的总RNA制备的cDNA与人类cDNA微阵列（1152个元件）杂交。通过SEM观察到的六种牙科基底材料表面外观各不相同。确定SLA和HA表面比其他表面更粗糙。在SLA和HA上培养的MG63细胞表现出细胞-基质相互作用。在骨整合相关基因的表达中，包括骨形态发生蛋白、钙黏蛋白、整合素和胰岛素样生长因子在内的几个基因在不同表面上上调。包括成纤维细胞生长因子受体4、Bcl-2相关蛋白和胶原蛋白在内的几个基因在不同表面上下调。所用牙科材料的表面粗糙度增强了关键成骨调节基因的附着和表达。
