Qu Zhe, Rausch-Fan Xiaohui, Wieland Marco, Matejka Michael, Schedle Andreas
Department of Periodontology, Bernhard Gottlieb University Clinic of Dentistry, Medical University of Vienna, Austria.
J Biomed Mater Res A. 2007 Sep 1;82(3):658-68. doi: 10.1002/jbm.a.31023.
The aim of this study was to investigate the influence of hydrophobic acid-etched (A) and coarse-blasted large-grit and acid-etched (SLA) surfaces as well as hydrophilic modified acid-etched (modA) and modified coarse-blasted large-grit and acid-etched (modSLA) surfaces on the behavior of MG63 cells grown on these surfaces through determination of cell attachment and cell proliferation, time-lapse microscopy of fluorescence-labeled cells, and determination of gene expression by reverse transcription-polymerase chain reaction (RT-PCR). No significant difference of cell attachment on various titanium surfaces was found. Increased cell proliferation was observed on the A surface and the SLA surface compared with the modA surface and the modSLA surface. After 2 days of incubation, on modSLA and modA surfaces a tendency of formation of cell clusters has been observed, which was most pronounced on modSLA surface. On the A and the SLA surface, cell cluster formation started after longer incubation periods. The expression level of the bone-associated genes (alkaline phosphatase, osteocalcin, type-I-collagen, osteoprotegerin, and glyceraldehyde-3-phosphate-dehydrogenase) detected by RT-PCR was highest on the modSLA surface. In conclusion it has been demonstrated that the modSLA surface results in an enhanced cluster formation of osteoblasts grown on this surface and in an increased expression of key osteogenic regulatory genes in osteoblasts.
本研究的目的是通过测定细胞附着和细胞增殖、荧光标记细胞的延时显微镜观察以及逆转录-聚合酶链反应（RT-PCR）测定基因表达，来研究疏水酸蚀（A）表面、粗喷砂大颗粒酸蚀（SLA）表面以及亲水改性酸蚀（modA）表面和改性粗喷砂大颗粒酸蚀（modSLA）表面对在这些表面上生长的MG63细胞行为的影响。在各种钛表面上未发现细胞附着有显著差异。与modA表面和modSLA表面相比，在A表面和SLA表面观察到细胞增殖增加。孵育2天后，在modSLA和modA表面观察到有细胞簇形成的趋势，在modSLA表面最为明显。在A和SLA表面，细胞簇形成在更长的孵育期后开始。通过RT-PCR检测的骨相关基因（碱性磷酸酶、骨钙素、I型胶原蛋白、骨保护素和甘油醛-3-磷酸脱氢酶）的表达水平在modSLA表面最高。总之，已证明modSLA表面可导致在该表面上生长的成骨细胞形成增强的细胞簇，并使成骨细胞中关键成骨调节基因的表达增加。
