Haschek B, Klein D, Benetka V, Herrera C, Sommerfeld-Stur I, Vilcek S, Moestl K, Baumgartner W
Clinic for Ruminants, Department for Farm Animals and Herd Management, University of Veterinary Medicine, Veterinaerplatz 1, A-1210 Vienna, Austria.
J Vet Med B Infect Dis Vet Public Health. 2006 May;53(4):160-5. doi: 10.1111/j.1439-0450.2006.00936.x.
Faeces of 230 calves with and without diarrhoea collected during the winter period 2004/2005 in 100 Austrian farms (Styria and Lower Austria) were examined for viral, bacterial and parasitic enteropathogens. Torovirus-specific nucleic acid confirmed by reverse transcriptase-polymerase chain reaction was found in 12 of 230 calves (5.2%). Ten of these calves were clinically ill, several of them showing signs of dehydration and abnormal faecal consistency at the time of sampling. Computer assisted analysis of two nucleotide sequences obtained from Austrian bovine samples revealed 93% similarity to Breda strain, but only 71% or 52% similarity to Equine Berne or Porcine Markelo torovirus strains respectively. Phylogenetic analysis grouped Austrian torovirus samples into the Bovine torovirus cluster indicating the first detection of Bovine torovirus in Austria. In addition, the following agents were detected in bovine faecal samples: Bovine coronavirus, 25.7%; Escherichia coli, 17%; Cryptosporidium spp., 11.7%; Eimeria spp., 10.4%; Rotavirus, 9.1%; Clostridium perfringens, 9.1% and Giardia spp., 6.1%. Salmonella spp. was not detected.
2004/2005年冬季期间，在奥地利100个农场（施泰尔马克州和下奥地利州）收集了230头有腹泻和无腹泻小牛的粪便，检测其中的病毒、细菌和寄生虫性肠道病原体。通过逆转录-聚合酶链反应确认，在230头小牛中有12头（5.2%）检测到了牛环曲病毒特异性核酸。其中10头小牛有临床症状，几头在采样时出现脱水迹象和粪便性状异常。对从奥地利牛样本中获得的两个核苷酸序列进行计算机辅助分析发现，其与布雷达毒株的相似度为93%，但与马伯尔尼环曲病毒株或猪马凯洛环曲病毒株的相似度分别仅为71%或52%。系统发育分析将奥地利环曲病毒样本归入牛环曲病毒簇，表明在奥地利首次检测到牛环曲病毒。此外，在牛粪便样本中还检测到了以下病原体：牛冠状病毒，25.7%；大肠杆菌，17%；隐孢子虫属，11.7%；艾美耳球虫属，10.4%；轮状病毒，9.1%；产气荚膜梭菌，9.1%；贾第虫属，6.1%。未检测到沙门氏菌属。
