Hoet Armando E, Nielsen Paul R, Hasoksuz Mustafa, Thomas Christopher, Wittum Thomas E, Saif Linda J
Food Animal Health Research Program, Department of Veterinary Preventive Medicine, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH 44691, USA.
J Vet Diagn Invest. 2003 May;15(3):205-12. doi: 10.1177/104063870301500301.
The objectives of this study were to determine the prevalence of bovine torovirus (BoTV) in bovine fecal samples from diarrhea cases submitted to the Ohio Animal Disease Diagnostic Laboratory (ADDL) and to assess if a relationship exists between BoTV and the other enteric pathogens detected. From November 1999 to May 2001, 259 specimens from 53 calves (< or = 6 months old), 27 young adults (52 years), 125 adults (> or = 2 years), and 54 animals of unknown age were examined by an antigen-capture enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase-polymerase chain reaction (RT-PCR) assay developed to detect BoTV. Testing for other enteric pathogens was performed by ADDL, and the results were analyzed with the BoTV data. The BoTV was detected using ELISA or RT-PCR in 9.7% (25/259) of the clinical samples, 56% (14/25) of which were from calves (P < 0.001) representing 26.4% (14/53) of the calves tested. Of the BoTV-positive calves, 71% (10/14) were less than 3 weeks of age. In 11/25 positive specimens, BoTV was the only pathogen detected among those examined. Other enteric organisms detected alone or in combination with BoTV in calf samples were rotavirus, coronavirus, Salmonella spp., Cryptosporidium spp., and Giardia spp.; but no consistent association between BoTV and these organisms was observed. In summary, BoTV was detected in fecal samples from cattle with diarrhea, principally in young calves less than 3 weeks of age. Future studies of infectious diarrhea in cattle should also include assays for this etiologic agent.
本研究的目的是确定提交至俄亥俄动物疾病诊断实验室（ADDL）的腹泻病例牛粪便样本中牛环曲病毒（BoTV）的流行率，并评估BoTV与检测到的其他肠道病原体之间是否存在关联。1999年11月至2001年5月，对来自53头犊牛（≤6月龄）、27头青年牛（5至2岁）、125头成年牛（≥2岁）和54头年龄未知动物的259份样本，采用为检测BoTV而开发的抗原捕获酶联免疫吸附测定（ELISA）和逆转录聚合酶链反应（RT-PCR）进行检测。ADDL对其他肠道病原体进行检测，并将结果与BoTV数据进行分析。在9.7%（25/259）的临床样本中通过ELISA或RT-PCR检测到BoTV，其中56%（14/25）来自犊牛（P<0.001），占检测犊牛的26.4%（14/53）。在BoTV阳性的犊牛中，71%（10/14）小于3周龄。在11/25份阳性样本中，BoTV是检测到的唯一病原体。在犊牛样本中单独或与BoTV一起检测到的其他肠道生物有轮状病毒、冠状病毒、沙门氏菌属、隐孢子虫属和贾第虫属；但未观察到BoTV与这些生物之间存在一致关联。总之，在腹泻牛的粪便样本中检测到BoTV，主要在小于3周龄的犊牛中。未来对牛传染性腹泻的研究也应包括对这种病原体的检测。
