Brink Hallie E, Miller Gregory J, Beredjiklian Pedro K, Nicoll Steven B
Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvannia 19104, USA.
Wound Repair Regen. 2006 Mar-Apr;14(2):179-86. doi: 10.1111/j.1743-6109.2006.00108.x.
Cell migration and extracellular matrix synthesis play an important role in the wound-healing response to injury. Several studies have described differences in migratory behavior and collagen biosynthetic activity in adult vs. fetal skin fibroblasts. The objective of this study was to examine the serum- and age-dependent effects on cell migration and collagen expression in tendon fibroblasts. Medial tendon fibroblasts were isolated from pregnant ewes and their fetuses, and cultured with and without serum for up to 7 days. Cell migration was determined by quantitative image analysis, and collagen expression was assessed by reverse transcription-polymerase chain reaction and immunohistochemical staining. In serum-containing medium, tendon fibroblasts migrated significantly faster than cells in serum-free medium. Additionally, fetal tendon fibroblasts migrated significantly faster than adult tendon fibroblasts under both culture conditions. The expression of types I and III collagen mRNA was significantly up-regulated in tendon cell populations in serum-free medium compared with those in serum-containing medium. Quantitative assessment of collagen staining indicated that fetal tenocytes produced more type I collagen than adult tenocytes under both culture conditions. These findings suggest that there is an inherent difference between adult and fetal tendon fibroblasts, which may have implications in the wound-healing response in tendons.
细胞迁移和细胞外基质合成在损伤后的伤口愈合反应中起着重要作用。多项研究描述了成年与胎儿皮肤成纤维细胞在迁移行为和胶原蛋白生物合成活性方面的差异。本研究的目的是检测血清和年龄对肌腱成纤维细胞迁移和胶原蛋白表达的影响。从怀孕母羊及其胎儿中分离出内侧肌腱成纤维细胞，并在有血清和无血清条件下培养长达7天。通过定量图像分析确定细胞迁移，通过逆转录-聚合酶链反应和免疫组织化学染色评估胶原蛋白表达。在含血清培养基中，肌腱成纤维细胞的迁移速度明显快于无血清培养基中的细胞。此外，在两种培养条件下，胎儿肌腱成纤维细胞的迁移速度均明显快于成年肌腱成纤维细胞。与含血清培养基中的肌腱细胞群体相比，无血清培养基中I型和III型胶原蛋白mRNA的表达明显上调。胶原蛋白染色的定量评估表明，在两种培养条件下，胎儿肌腱细胞产生的I型胶原蛋白均多于成年肌腱细胞。这些发现表明，成年和胎儿肌腱成纤维细胞之间存在内在差异，这可能对肌腱的伤口愈合反应有影响。
