Packer D L, Dombi G W, Yu P Y, Zidel P, Sullivan W G
Department of Surgery, Wayne State University, Detroit, MI 48201.
J Hand Surg Am. 1994 Sep;19(5):769-76. doi: 10.1016/0363-5023(94)90181-3.
We studied fibroblast activity during tendon healing with an in vitro tendon culture model. Tendons were embedded in a translucent collagen gel matrix whose porous nature permitted free nutrient diffusion, fibroblast migration out of the tendon, and microphotographic documentation of fibroblast activity. Experiments were performed using one or more tendons cultured in the same collagen gel. We identified three zones of fibroblast activity in the gel. Zone I was an area of randomly dispersed cells directly adjacent to the tendon where collagen synthesis and remodeling were probably taking place. In zone II, spindle-shaped fibroblasts were aligned pointing away from the cut tendon end forming a sunburst-like aggregate of cells. Zone II fibroblasts were responsible for formation of migration trails by exerting a mechanical force on the collagen matrix, which was evident as a local gel contraction. Zone III was the leading edge of the sunburst populated by the fastest moving fibroblasts, which responded to guidance by other cut tendon ends. We speculate that the collagen gel used in the culture system may help maintain a chemotactic concentration gradient that allows fibroblasts to locate other distal cut tendon surfaces also embedded in the collagen gel.
我们使用体外肌腱培养模型研究了肌腱愈合过程中的成纤维细胞活性。将肌腱嵌入半透明的胶原凝胶基质中，该基质的多孔性质允许营养物质自由扩散、成纤维细胞从肌腱中迁移出来，并对成纤维细胞活性进行显微照相记录。实验使用在同一胶原凝胶中培养的一根或多根肌腱进行。我们在凝胶中确定了三个成纤维细胞活性区域。区域I是紧邻肌腱的随机分散细胞区域，胶原合成和重塑可能在此发生。在区域II中，纺锤形的成纤维细胞排列成远离切断肌腱末端的方向，形成类似旭日状的细胞聚集体。区域II的成纤维细胞通过对胶原基质施加机械力来负责形成迁移轨迹，这表现为局部凝胶收缩。区域III是旭日状的前沿，由移动最快的成纤维细胞组成，它们对其他切断的肌腱末端的引导做出反应。我们推测，培养系统中使用的胶原凝胶可能有助于维持趋化浓度梯度，使成纤维细胞能够定位同样嵌入胶原凝胶中的其他远端切断肌腱表面。
