NEC8人胚胎癌细胞分化过程中的DMRT-1表达。
DMRT-1 expression during NEC8 human embryonic carcinoma cell differentiation.
作者信息
Koji Hitoshi, Yamada Akira, Nagasawa Toshihiko, Gamou Shinobu
机构信息
Department of Environmental and Life Sciences, Kyorin University School of Health Sciences, 876 Miyashita-cho, Hachhioji, Tokyo 192-8508, Japan.
出版信息
Cancer Sci. 2006 Apr;97(4):277-82. doi: 10.1111/j.1349-7006.2006.00178.x.
To elucidate the relationship between dsx and mab-3 related transcription factor 1 (Dmrt-1) and differentiation, alteration in mRNA levels during differentiation of NEC8 human embryonic carcinoma cells was investigated. After stimulation with 50 nM phorbol 12-myristate 13-acetate (PMA), the cells differentiated into cells with mesenchymal characteristics and upregulated Dmrt-1 mRNA, possibly through the protein kinase C/mitogen-activated protein kinase/activated protein-1 signaling pathway. Conversely, knockdown of Dmrt-1 by small interfering RNA resulted in cell morphology that was different from that after PMA treatment. These results indicated that Dmrt-1 expression was apparently associated with the differentiation of NEC8, and this cell line may be a helpful in vitro tool to clarify the role of Dmrt-1 in the differentiation process.
为阐明双性基因（dsx）与 mab-3 相关转录因子 1（Dmrt-1）之间的关系以及它们与分化的关系，研究了 NEC8 人胚胎癌细胞分化过程中 mRNA 水平的变化。用 50 nM 佛波酯 12-肉豆蔻酸酯 13-乙酸酯（PMA）刺激后，细胞分化为具有间充质特征的细胞，并上调了 Dmrt-1 mRNA，这可能是通过蛋白激酶 C/丝裂原活化蛋白激酶/活化蛋白-1 信号通路实现的。相反，用小干扰 RNA 敲低 Dmrt-1 导致细胞形态与 PMA 处理后的不同。这些结果表明，Dmrt-1 的表达显然与 NEC8 的分化相关，并且该细胞系可能是阐明 Dmrt-1 在分化过程中作用的有用体外工具。
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