Piboonpocanun S, Malainual N, Jirapongsananuruk O, Vichyanond P, Thomas W R
Institute of Molecular Biology and Genetics, Mahidol University, Nakhon Pathom, Thailand.
Clin Exp Allergy. 2006 Apr;36(4):510-6. doi: 10.1111/j.1365-2222.2006.02464.x.
Polymorphic sequence substitutions in the major mite allergens can markedly affect immunoglobulin E binding and T cell responses, but there are few studies on environmental isolates from Dermatophagoides pteronyssinus and none for D. farinae.
To determine the sequence variation of the group 1 and 2 allergens from environmental D. pteronyssinus and D. farinae.
RNA from each species was isolated from homes in Bangkok and the sequence of Der p 1, Der p 2, Der f 1, and Der f 2 determined from cDNA produced by high fidelity polymerase chain reactions.
The enlarged data set revealed preferred amino acid substitutions in residues 19, 81, and 215 of Der p 1 as well as sporadic changes. Der p 2 showed frequent variations with clusters of amino acid substitutions, but the canonical Der p 2.0101 was not found in any of 17 sequences. Der f 2 showed variants with clusters of substitutions similar to Der p 2 but in different amino acid positions and without any structural concordance. Der f 1 in contrast to the other allergens had few amino acid sequence substitutions.
The sequence information on variants provides data important for the optimal design of allergen formulations and useful for the genetic engineering and structure-function analyses of the major allergens.
主要螨类变应原中的多态性序列替换可显著影响免疫球蛋白E结合及T细胞反应，但关于来自屋尘螨的环境分离株的研究较少，而对于粉尘螨则尚无相关研究。
确定来自环境中的屋尘螨和粉尘螨1组及2组变应原的序列变异情况。
从曼谷家庭中采集每个物种的RNA，通过高保真聚合酶链反应从cDNA中确定Der p 1、Der p 2、Der f 1和Der f 2的序列。
扩大后的数据集显示，Der p 1的第19、81和215位残基存在优先氨基酸替换以及散在变化。Der p 2显示出频繁变异且存在氨基酸替换簇，但在17个序列中的任何一个中均未发现典型的Der p 2.0101。Der f 2显示出与Der p 2类似的具有替换簇的变体，但氨基酸位置不同且无任何结构一致性。与其他变应原相比，Der f 1的氨基酸序列替换较少。
变异体的序列信息为变应原制剂的优化设计提供了重要数据，对主要变应原的基因工程及结构-功能分析也很有用。
