Sørensen Kristine Bjørndal, Godballe Christian, de Stricker Karin, Krogdahl Annelise
Department of Pathology, Odense University Hospital, Odense, Denmark.
J Oral Pathol Med. 2006 May;35(5):286-91. doi: 10.1111/j.1600-0714.2006.00421.x.
Our aim is to investigate the expression of kit protein (KIT) and epidermal growth factor receptor (EGFR) in parotid carcinomas in order to correlate the expression to histology and prognosis. Further we want to perform mutation analysis of KIT-positive adenoid cystic carcinomas.
Formalin-fixed paraffin-embedded sections from 73 patients with parotid gland carcinomas were used for the study. The sections were stained with both KIT and EGFR polyclonal antibodies. Twelve KIT-positive adenoid cystic carcinomas were examined for c-kit mutation in codon 816.
Of all carcinomas 25% were KIT-positive and 79% were EGFR-positive. Ninety-two percentage of the adenoid cystic carcinomas were KIT-positive. None of the adenoid cystic carcinomas had mutations in codon 816 of the c-kit gene.
Neither KIT- nor EGFR-expression seem to harbour significant prognostic information. Adenoid cystic carcinomas express KIT, but no mutations in codon 816 of the c-kit gene were identified.
我们的目的是研究腮腺癌中kit蛋白（KIT）和表皮生长因子受体（EGFR）的表达情况，以便将其表达与组织学和预后相关联。此外，我们还想对KIT阳性的腺样囊性癌进行突变分析。
本研究使用了73例腮腺癌患者的福尔马林固定石蜡包埋切片。切片用KIT和EGFR多克隆抗体染色。对12例KIT阳性的腺样囊性癌进行第816密码子c-kit突变检测。
在所有癌中，25%为KIT阳性，79%为EGFR阳性。92%的腺样囊性癌为KIT阳性。所有腺样囊性癌均未检测到c-kit基因第816密码子的突变。
KIT和EGFR的表达似乎均不具有显著的预后信息。腺样囊性癌表达KIT，但未发现c-kit基因第816密码子的突变。
