Wu T, Lundgren J D, Rieves R D, Doerfler M E, Logun C, Shelhamer J H
Department of Critical Care Medicine, National Institutes of Health, Bethesda, MD 20892.
Exp Lung Res. 1991 Nov-Dec;17(6):1079-94. doi: 10.3109/01902149109064336.
Eicosanoids have been shown to be major mediators of airway inflammation. Platelet-activating factor (PAF), a potent bronchoconstrictor and stimulator of respiratory mucous secretion, may mediate some of its effects via eicosanoid production. To explore eicosanoid generation by cultured feline tracheal explants, eicosanoids were measured following PAF stimulation. After labeling the explants with [3H]arachidonic acid, supernatant from control and PAF treated explants was fractionated by reverse phase high-performance liquid chromatography (HPLC). The resulting elution pattern suggested the release of arachidonic acid (AA), 15-hydroxyeicosatetraenoic acid (HETE), leukotriene (LT)B4, C4, prostaglandin (PG) D2/E2/F2 alpha, and 6-keto-PGF1 alpha. Radioimmunoassay (RIA) following HPLC resolution confirmed that PAF induced a significantly increased release of peptido-leukotrienes, PGD2, PGE2, PGF2 alpha, LTB4, and 5-HETE, as well as thromboxane (TX) B2. The most remarkable increase was LTC4/D4/E4 (15 x control) and PGD2 (4 x control). The PAF antagonist Ro 19-3704 had an inhibitory effect on the PAF-stimulated release of peptido-leukotrienes. We conclude that PAF stimulates the production of a variety of lipoxygenase and cyclooxygenase pathway metabolites in feline airways.
类二十烷酸已被证明是气道炎症的主要介质。血小板活化因子（PAF）是一种强效支气管收缩剂和呼吸道黏液分泌刺激剂，可能通过类二十烷酸的产生介导其部分作用。为了探究培养的猫气管外植体产生类二十烷酸的情况，在PAF刺激后对类二十烷酸进行了测量。在用[3H]花生四烯酸标记外植体后，通过反相高效液相色谱（HPLC）对对照和PAF处理的外植体的上清液进行分离。所得洗脱图谱表明释放了花生四烯酸（AA）、15-羟基二十碳四烯酸（HETE）、白三烯（LT）B4、C4、前列腺素（PG）D2/E2/F2α和6-酮-PGF1α。HPLC分离后的放射免疫测定（RIA）证实，PAF诱导肽白三烯、PGD2、PGE2、PGF2α、LTB4和5-HETE以及血栓素（TX）B2的释放显著增加。最显著的增加是LTC4/D4/E4（15倍于对照）和PGD2（4倍于对照）。PAF拮抗剂Ro 19-3704对PAF刺激的肽白三烯释放具有抑制作用。我们得出结论，PAF刺激猫气道中多种脂氧合酶和环氧化酶途径代谢产物的产生。
