Yzquierdo Sergio Luis, Lemus Dihadenys, Echemendia Miguel, Montoro Ernesto, McNerney Ruth, Martin Anandi, Palomino Juan Carlos
Instituto de Medicina Tropical Pedro Kouri, La Habana, Cuba.
Ann Clin Microbiol Antimicrob. 2006 Apr 21;5:11. doi: 10.1186/1476-0711-5-11.
Conventional methods for susceptibility testing require several months before results can be reported. However, rapid methods to determine drug susceptibility have been developed recently. Phage assay have been reported as a rapid useful tools for antimicrobial susceptibility testing. The aim of this study was to apply the Phage assay for rapid detection of resistance on Mycobacterium tuberculosis strains in Cuba.
Phage D29 assay was performed on 102 M. tuberculosis strains to detect rifampicin resistance. The results were compared with the proportion method (gold standard) to evaluate the sensitivity and specificity of Phage assay.
Phage assay results were available in 2 days whereas Proportion Methods results were obtain in 42 days. A total of 44 strains were detected as rifampicin resistant by both methods. However, one strains deemed resistant by Proportion Methods was susceptible by Phage assay. The sensitivity and specificity of Phage assay were 97.8 % and 100% respectively.
Phage assay provides rapid and reliable results for susceptibility testing; it's easy to perform, requires no specialized equipment and is applicable to drug susceptibility testing in low income countries where tuberculosis is a major public health problem.
传统的药敏试验方法需要数月才能报告结果。然而，近年来已开发出快速确定药物敏感性的方法。噬菌体试验已被报道为抗菌药敏试验的一种快速有用工具。本研究的目的是应用噬菌体试验快速检测古巴结核分枝杆菌菌株的耐药性。
对102株结核分枝杆菌菌株进行噬菌体D29试验以检测利福平耐药性。将结果与比例法（金标准）进行比较，以评估噬菌体试验的敏感性和特异性。
噬菌体试验结果在2天内可得，而比例法结果在42天内获得。两种方法共检测出44株对利福平耐药的菌株。然而，比例法判定为耐药的1株菌株经噬菌体试验检测为敏感。噬菌体试验的敏感性和特异性分别为97.8%和100%。
噬菌体试验为药敏试验提供了快速可靠的结果；它易于操作，不需要专门设备，适用于结核病是主要公共卫生问题的低收入国家的药敏试验。
