Gangoué-Piéboji J, Pegnyemb D E, Niyitegeka D, Nsangou A, Eze N, Minyem C, Mbing J Ngo, Ngassam P, Tih R Ghogomu, Sodengam B L, Bodo B
Centre for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plant Studies, P.O. Box 8404, Yaoundé, Cameroon.
Ann Trop Med Parasitol. 2006 Apr;100(3):237-43. doi: 10.1179/136485906X86365.
The antimicrobial activities of 10 plant species (Voacanga africana, Crepis cameroonica, Plagiostyles africana, Crotalaria retusa, Mammea africana, Lophira lanceolata, Ochna afzelii, Ouratea elongata, Ou. flava and Ou. sulcata), each of which is currently used in the traditional medicine of Cameroon, were investigated in vitro. The activities of a methanol extract of each plant were tested, in disc-diffusion assays, against 37 reference or laboratory strains of seven species of microorganism (Staphylococcus aureus, S. epidermidis, Enterococcus hirae, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Candida albicans). The minimal inhibitory concentrations of each extract were then estimated, against each of the more susceptible microorganisms (i.e. those giving an inhibition zone measuring at least 9 mm in diameter in the disc-diffusion assays), by agar dilution. Although, in the disc-diffusion assays, each of the 10 methanol extracts investigated displayed some degree of antimicrobial activity against at least one species of microorganism, no activity against the Gram-negative bacteria (Es. coli, K. pneumoniae and Ps. aeruginosa) was observed. The extract with the greatest antimicrobial activity was that of Pl. africana (Euphorbiaceae).
对喀麦隆传统医学中目前使用的10种植物（非洲沃坎加、喀麦隆还阳参、非洲斜柱榄、猪屎豆、非洲牛油果、披针叶风车子、阿氏金莲木、长叶金莲木、黄果金莲木和槽叶金莲木）的抗菌活性进行了体外研究。在纸片扩散试验中，测试了每种植物甲醇提取物对7种微生物（金黄色葡萄球菌、表皮葡萄球菌、海氏肠球菌、大肠杆菌、肺炎克雷伯菌、铜绿假单胞菌和白色念珠菌）的37株参考菌株或实验室菌株的活性。然后通过琼脂稀释法，针对每种更敏感的微生物（即在纸片扩散试验中产生直径至少为9毫米抑菌圈的微生物）估计每种提取物的最低抑菌浓度。虽然在纸片扩散试验中，所研究的10种甲醇提取物中的每一种都对至少一种微生物表现出一定程度的抗菌活性，但未观察到对革兰氏阴性菌（大肠杆菌、肺炎克雷伯菌和铜绿假单胞菌）的活性。抗菌活性最强的提取物是非洲斜柱榄（大戟科）的提取物。
