Liu Xiaodong, Pohl Christopher A, Weiss Joachim
Dionex Corporation, Sunnyvale, CA 94088, USA.
J Chromatogr A. 2006 Jun 16;1118(1):29-34. doi: 10.1016/j.chroma.2006.03.080. Epub 2006 Apr 19.
The separation and identification of surfactants is a challenge due to the diversity of surfactants, the complex composition of surfactant raw materials, and the complexity of the sample matrices. High-performance liquid chromatography (HPLC) is the preferred analytical technique, because it allows the characterization of surfactant raw materials according to their composition and the quantitation of individual surfactants in complex mixtures. Although many HPLC columns are available for surfactant analysis, none of these columns provide optimal selectivity for the simultaneous analysis of anionic, non-ionic, and cationic surfactants using the same mobile phase system. In this paper, we describe a new polar-embedded stationary phase for the simultaneous analysis of anionic, non-ionic, and cationic surfactants with a simple and volatile mobile phase system containing ammonium acetate buffer and acetonitrile, utilizing evaporative light-scattering detection (ELSD). Mobile phase ionic strength and pH are important for optimizing chromatographic conditions. The column offers ideal selectivity for different types of surfactants, excellent peak shapes, especially for cationic surfactants, improved resolution for oligomers in ethoxylated surfactants, and compatibility with highly aqueous mobile phases. Thus, it can be used not only for quality assurance of individual surfactants, but also for the analysis of a variety of surfactant-containing formulations.
由于表面活性剂种类繁多、表面活性剂原料组成复杂以及样品基质的复杂性，表面活性剂的分离和鉴定具有挑战性。高效液相色谱法（HPLC）是首选的分析技术，因为它能够根据表面活性剂原料的组成对其进行表征，并对复杂混合物中的单个表面活性剂进行定量分析。尽管有许多HPLC柱可用于表面活性剂分析，但这些柱中没有一种能在使用相同流动相系统的情况下，对阴离子、非离子和阳离子表面活性剂的同时分析提供最佳选择性。在本文中，我们描述了一种新型的极性嵌入固定相，用于同时分析阴离子、非离子和阳离子表面活性剂，该固定相采用含有醋酸铵缓冲液和乙腈的简单挥发性流动相系统，并利用蒸发光散射检测（ELSD）。流动相的离子强度和pH值对于优化色谱条件很重要。该柱对不同类型的表面活性剂具有理想的选择性、出色的峰形，特别是对阳离子表面活性剂，提高了乙氧基化表面活性剂中低聚物的分离度，并且与高水相流动相兼容。因此，它不仅可用于单个表面活性剂的质量保证，还可用于分析各种含表面活性剂的配方。
