Namimatsu A, Go K, Hata T
Institute of Bio-Active Science, Nippon Zoki Pharmaceutical Company, Hyogo, Japan.
Int Arch Allergy Appl Immunol. 1991;96(2):107-12. doi: 10.1159/000235480.
To develop an animal model for experimental nasal hypersensitivity and hyperreactivity, guinea pigs were subjected to intermittent exposure to cold temperature (intermittent cold stress, SART stress) for 5 consecutive days. In SART-stressed guinea pigs, nasal mucosal hypersensitivity to histamine evoking sneeze response and nasal hypersecretion in response to methacholine were observed. The hypersensitivity remained for further 7 days after being released from SART stress. On the other hand, such nasal mucosal hypersensitivity was not caused by a continuous cold stress alone, suggesting that intermittent exposure to cold may be of importance for the appearance of nasal mucosal hypersensitivity. In passively sensitized SART-stressed guinea pigs, the quantity of nasal secretion induced by an allergen was significantly increased compared with that of a group of normal animals. The expression of muscarinic acetylcholine receptor (m-ACh.R) became higher in SART-stressed guinea pigs. Thus, hypersensitivity and hyperreactivity in this system were found to be associated with an increase in density of m-ACh.R. SART-stressed guinea pigs will serve as an animal model for hypersensitivity in nasal mucosa, which would be useful for the study of nasal allergy.
为建立实验性鼻超敏反应和高反应性的动物模型，豚鼠连续5天接受间歇性低温暴露（间歇性冷应激，SART应激）。在SART应激的豚鼠中，观察到鼻黏膜对组胺诱发喷嚏反应的超敏反应以及对乙酰甲胆碱的鼻分泌亢进。从SART应激中释放后，这种超敏反应持续7天。另一方面，单纯持续冷应激不会引起这种鼻黏膜超敏反应，提示间歇性冷暴露可能对鼻黏膜超敏反应的出现至关重要。在被动致敏的SART应激豚鼠中，与正常动物组相比，变应原诱导的鼻分泌物量显著增加。SART应激豚鼠中毒蕈碱型乙酰胆碱受体（m-ACh.R）的表达升高。因此，发现该系统中的超敏反应和高反应性与m-ACh.R密度增加有关。SART应激豚鼠将作为鼻黏膜超敏反应的动物模型，这将有助于鼻过敏的研究。
