Sliva Katja, Schnierle Barbara S
Institute for Biomedical Research, Georg-Speyer-Haus, Paul-Ehrlich-Str. 42-44, 60596 Frankfurt/Main, Germany.
Virology. 2006 Jul 20;351(1):218-25. doi: 10.1016/j.virol.2006.03.014. Epub 2006 May 2.
Short hairpin RNA (shRNA) can be stably expressed in cells to down-modulate gene expression. While retroviral and lentiviral vectors can be used to deliver shRNAs, the restricted viral titer is the major limitation for efficient gene transfer, which is especially important for cancer gene therapy. We were interested in using replicating murine leukemia virus (MLV) to enhance the shRNA transfer. Although stem loop structures could potentially interfere with the retroviral life cycle, we were able to demonstrate that the insertion of shRNA expression cassettes into MLV did not interfere significantly with viral fitness. The virus was genetically stable and able to silence target gene expression. Our results show that replicating MLVs are excellent tools for delivering shRNAs efficiently throughout the culture and have the potential to be used for gene function elucidation or even for cancer gene therapy.
短发夹RNA（shRNA）可在细胞中稳定表达以下调基因表达。虽然逆转录病毒和慢病毒载体可用于递送shRNAs，但有限的病毒滴度是有效基因转移的主要限制，这对癌症基因治疗尤为重要。我们有兴趣使用复制型鼠白血病病毒（MLV）来增强shRNA的转移。尽管茎环结构可能会干扰逆转录病毒的生命周期，但我们能够证明将shRNA表达盒插入MLV不会显著干扰病毒适应性。该病毒基因稳定，能够沉默靶基因表达。我们的结果表明，复制型MLV是在整个培养过程中有效递送shRNAs的优秀工具，并且有潜力用于基因功能阐明甚至癌症基因治疗。
