Wang Bin, Zhao Yanhong, Lin Hang, Chen Bing, Zhang Jing, Zhang Jingyu, Wang Xia, Zhao Wenxue, Dai Jianwu
Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 3 Nanyitiao, Zhongguancun, Beijing 100080, China.
Neurosci Lett. 2006 Jun 19;401(1-2):65-70. doi: 10.1016/j.neulet.2006.02.085. Epub 2006 Apr 24.
Olfactory ensheathing cell (OEC) transplantation is a promising or potential therapy for spinal cord injury (SCI). However, the effects of injecting OECs directly into SCI site have been limited and unsatisfied due to the complexity of SCI. To improve the outcome, proper biomaterials are thought to be helpful since these materials would allow the cells to grow three-dimensionally and guide cell migration. In this paper, we have studied the behavior of OECs in two-dimensional (2-D) condition as well as on three-dimensional (3-D) collagen scaffolds by analyzing their phenotypes such as cell proliferation, apoptosis, morphology, and gene activities of some neurotrophic factors and myelin proteins. OECs proliferation rate was increased on 3-D collagen scaffolds compared to the 2-D culture condition. OECs on 3-D collagen scaffolds also showed less apoptosis. In addition, OECs on 3-D collagen scaffolds maintained the original spindle-shape morphology and P75NTR gene activity. NGF, BDNF, and PLP were found to be upregulated in OECs cultured on 3-D collagen scaffolds by the semi-quantitative RT-PCR approach. The results suggested that 3-D collagen scaffolds provide suitable environments for the OECs to maintain their morphology as well as several important functional phenotypes and all these could be helpful for the effective treatment of SCI.
嗅鞘细胞（OEC）移植是一种有前景的或潜在的脊髓损伤（SCI）治疗方法。然而，由于脊髓损伤的复杂性，将OEC直接注射到脊髓损伤部位的效果有限且不尽人意。为了改善治疗效果，合适的生物材料被认为是有帮助的，因为这些材料能够使细胞进行三维生长并引导细胞迁移。在本文中，我们通过分析嗅鞘细胞的表型，如细胞增殖、凋亡、形态以及一些神经营养因子和髓磷脂蛋白的基因活性，研究了嗅鞘细胞在二维（2-D）条件下以及在三维（3-D）胶原支架上的行为。与二维培养条件相比，嗅鞘细胞在三维胶原支架上的增殖率有所提高。三维胶原支架上的嗅鞘细胞凋亡也较少。此外，三维胶原支架上的嗅鞘细胞保持了原始的纺锤形形态和P75NTR基因活性。通过半定量逆转录聚合酶链反应（RT-PCR）方法发现，在三维胶原支架上培养的嗅鞘细胞中神经生长因子（NGF）、脑源性神经营养因子（BDNF）和髓磷脂蛋白脂蛋白（PLP）的表达上调。结果表明，三维胶原支架为嗅鞘细胞提供了合适的环境，以维持其形态以及几种重要的功能表型，所有这些都有助于脊髓损伤的有效治疗。
