Manfroid Isabelle, Caubit Xavier, Marcelle Christophe, Fasano Laurent
Institut de Biologie du Développement de Marseille-Luminy, UMR CNRS 6216, Centre National de la Recherche Scientifique-Université de la Méditerranée, Campus de Luminy, F-13288 Marseille cedex 09, France.
Gene Expr Patterns. 2006 Oct;6(8):908-12. doi: 10.1016/j.modgep.2006.03.004. Epub 2006 May 2.
Drosophila teashirt (tsh) is involved in the patterning of the trunk identity together with the Hox genes. In addition, it is also a player in the Wingless and the Hedgehog pathways. In birds and mammals, three Tshz genes are identified and the expression patterns for mouse Tshz1 and Tshz2 have been reported during embryogenesis. Recently, we showed that all three mouse Tshz genes can rescue the Drosophila tsh loss-of-function phenotype, indicating that the function of the teashirt genes has been conserved during evolution. Here we describe the expression pattern of chick TSHZ3 during embryogenesis. Chick TSHZ3 is expressed in several tissues including mesodermal derivatives, the central and peripheral nervous systems. Emphasis is laid on the dynamic expression occurring in regions of the somites and limbs where tendons develop. We show that TSHZ3 is activated in the somites by FGF8, a known inducer of the tendon marker SCX.
果蝇的无尾基因（tsh）与同源异型基因一起参与躯体特征模式的形成。此外，它还在无翅和刺猬信号通路中发挥作用。在鸟类和哺乳动物中，已鉴定出三个Tshz基因，并且在胚胎发育过程中已报道了小鼠Tshz1和Tshz2的表达模式。最近，我们发现所有三个小鼠Tshz基因都可以挽救果蝇无尾基因功能丧失的表型，这表明无尾基因的功能在进化过程中得以保留。在此，我们描述了鸡TSHZ3在胚胎发育过程中的表达模式。鸡TSHZ3在包括中胚层衍生物、中枢和外周神经系统在内的多种组织中表达。重点关注在肌腱发育的体节和肢体区域中发生的动态表达。我们发现TSHZ3在体节中被FGF8激活，FGF8是一种已知的肌腱标记物SCX的诱导剂。
