Heinemann Fabian, Treccani Laura, Fritz Monika
Biophysics Institute, University of Bremen, Otto-Hahn Allee, Germany.
Biochem Biophys Res Commun. 2006 May 26;344(1):45-9. doi: 10.1016/j.bbrc.2006.03.150. Epub 2006 Mar 31.
Mollusc shell formation takes place in a preformed extracellular matrix, composed of insoluble chitin, coated with proteins and dissolved macromolecules. The water-soluble matrix is known to have a strong influence on the growth of CaCO(3), whereas the role of the insoluble matrix on mineralization is unclear. Therefore, we mineralized the EDTA (ethylenediaminetetraacetic acid) insoluble organic matrix of abalone nacre with a modified double-diffusion set-up, where the diffusing solutions were constantly renewed. Control experiments were performed with cellulose and chitosan foils. The mineralized matrices/foils were analyzed with SEM. We show that the insoluble matrix of abalone nacre induces the growth of flat and roughly polygonal CaCO(3) crystals. In some of the experiments with the insoluble matrix, the growth of three-dimensional parallel sheets of densely packed platelets inside the insoluble matrix was observed. XRD on these samples revealed that they consist of oriented aragonite.
软体动物贝壳的形成发生在一个预先形成的细胞外基质中，该基质由不溶性几丁质组成，并覆盖有蛋白质和溶解的大分子。已知水溶性基质对碳酸钙（CaCO₃）的生长有强烈影响，而不溶性基质在矿化过程中的作用尚不清楚。因此，我们使用改良的双扩散装置对鲍鱼珍珠层的乙二胺四乙酸（EDTA）不溶性有机基质进行矿化，其中扩散溶液不断更新。用纤维素和壳聚糖箔进行对照实验。用扫描电子显微镜（SEM）分析矿化的基质/箔。我们表明，鲍鱼珍珠层的不溶性基质诱导扁平且大致呈多边形的碳酸钙晶体生长。在一些使用不溶性基质的实验中，观察到在不溶性基质内部有三维平行排列的紧密堆积血小板片层生长。对这些样品进行的X射线衍射（XRD）分析表明，它们由取向文石组成。
