人类蓖麻蛋白（Castor）的分子克隆与特性分析，蓖麻蛋白是一种在细胞分化过程中上调表达的新型人类基因。

Molecular cloning and characterization of human Castor, a novel human gene upregulated during cell differentiation.

作者信息

Liu Zhihui, Yang Xuezhong, Tan Fei, Cullion Kathleen, Thiele Carol J

机构信息

Cell and Molecular Biology Section, Pediatric Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.

出版信息

Biochem Biophys Res Commun. 2006 Jun 9;344(3):834-44. doi: 10.1016/j.bbrc.2006.03.207. Epub 2006 Apr 19.

DOI:10.1016/j.bbrc.2006.03.207

PMID:16631614

Abstract

Castor is a zinc finger transcription factor that controls cell fate within neuroblast cell lineages in Drosophila melanogaster. Here, we describe the cloning and characterization of a human castor gene (CASZ1) that is structurally homologous to Drosophila castor. We find the expression of castor gene is increased when cells of neural origin as well as mesenchymal origin are induced to differentiation. CASZ1 is expressed in a number of normal tissues and exists in at least two mRNA species of 4.4 and 8.0kb. They are named hCasz5 and hCasz11 because the predicted proteins have 5 and 11 zinc fingers, respectively. Deletion analysis of the proximal 5'-flanking sequences delineates sequences sufficient to drive transcription in cells of neural and non-neural origin. Both hCasz5 and hCasz11 localize predominantly in the nucleus, consistent with their role as Zn-finger containing transcription factor. CASZ1 is expressed in a number of human tumors and localizes to a chromosomal region frequently lost in tumors of neuroectodermal origin.

摘要

蓖麻毒素是一种锌指转录因子，可控制黑腹果蝇神经母细胞谱系中的细胞命运。在此，我们描述了一种与果蝇蓖麻毒素结构同源的人类蓖麻毒素基因（CASZ1）的克隆和特性。我们发现，当神经源性和间充质源性细胞被诱导分化时，蓖麻毒素基因的表达会增加。CASZ1在多种正常组织中表达，并且至少存在4.4kb和8.0kb的两种mRNA种类。它们分别被命名为hCasz5和hCasz11，因为预测的蛋白质分别具有5个和11个锌指。对近端5'侧翼序列的缺失分析确定了足以驱动神经源性和非神经源性细胞转录的序列。hCasz5和hCasz11都主要定位于细胞核，这与其作为含锌指转录因子的作用一致。CASZ1在多种人类肿瘤中表达，并定位于神经外胚层起源肿瘤中经常缺失的染色体区域。