Haratake M, Hidaka S, Ono M, Nakayama M
Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852-8521, Japan.
J Colloid Interface Sci. 2006 Jul 15;299(2):924-7. doi: 10.1016/j.jcis.2006.03.017. Epub 2006 May 2.
In this paper, we report on the preparation of an ionic polymer bead-supported lipid system several hundred micrometers in diameter. The electrostatic attractive interactions between anionic lipids and cationic polymer beads served as a "molecular glue" to immobilize the lipids on the beads, and then the immobilized lipids prompted the spontaneous formation of lipid bilayer membranes. Confocal fluorescence microscopic techniques revealed that the lipid bilayer membranes were located along the outline of the beads. In addition, the integrity of the lipid bilayer membranes was microscopically confirmed by a low-molecular-weight dye (trypan blue) exclusion test.
在本文中，我们报道了一种直径为几百微米的离子聚合物珠负载脂质体系的制备方法。阴离子脂质与阳离子聚合物珠之间的静电吸引相互作用充当“分子胶水”，将脂质固定在珠子上，然后固定化的脂质促使脂质双层膜的自发形成。共聚焦荧光显微镜技术显示脂质双层膜位于珠子的轮廓沿线。此外，通过低分子量染料（台盼蓝）排斥试验在显微镜下证实了脂质双层膜的完整性。
