Priglinger Siegfried G, May Christian-Albrecht, Alge Claudia S, Wolf Armin, Neubauer Aljoscha S, Haritoglou Christos, Kampik Anselm, Welge-Lussen Ulrich
Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany.
Cornea. 2006 Apr;25(3):331-5. doi: 10.1097/01.ico.0000183535.99651.7b.
To compare immunohistochemical findings in human donor corneas after successful laser in situ keratomileusis (LASIK) without clinical complications with a recently established human LASIK in vitro model.
Donor corneas with prior LASIK treatment were investigated. Cryostat sections were stained immunohistochemically for collagen types I, III, and VI and laminin and fibronectin.
With light microscopy, the interface of the LASIK flap could hardly be detected. In all samples, fibronectin was consistently detected along the entire extent of the surgical wound. In contrast, collagen type III and laminin only stained the superficial portion of the LASIK incision site. Staining for collagen types I and VI showed no changes after LASIK.
Histologic findings in donor corneas with prior LASIK treatment confirm histologic observations in a recently introduced human organ culture LASIK model. This strengthens the reliability of the latter LASIK model for further studies concerning wound healing after LASIK surgery.
将无临床并发症的成功准分子原位角膜磨镶术（LASIK）后人类供体角膜的免疫组化结果与最近建立的人类LASIK体外模型进行比较。
对先前接受LASIK治疗的供体角膜进行研究。冰冻切片进行免疫组化染色，检测I型、III型和VI型胶原以及层粘连蛋白和纤连蛋白。
通过光学显微镜，几乎无法检测到LASIK瓣的界面。在所有样本中，沿手术伤口的整个范围均持续检测到纤连蛋白。相比之下，III型胶原和层粘连蛋白仅对LASIK切口部位的表层进行染色。I型和VI型胶原染色在LASIK后未显示变化。
先前接受LASIK治疗的供体角膜的组织学结果证实了最近引入的人类器官培养LASIK模型中的组织学观察结果。这增强了后一种LASIK模型在LASIK手术后伤口愈合进一步研究中的可靠性。
