飞蝗利尿肽的特性分析

Characterization of a diuretic peptide from Locusta migratoria.

作者信息

Kay I, Wheeler C H, Coast G M, Totty N F, Cusinato O, Patel M, Goldsworthy G J

机构信息

Department of Biology, Birkbeck College, University of London, U.K.

出版信息

Biol Chem Hoppe Seyler. 1991 Oct;372(10):929-34. doi: 10.1515/bchm3.1991.372.2.929.

DOI:10.1515/bchm3.1991.372.2.929

PMID:1663363

Abstract

A diuretic peptide Locusta-DP, identified by its ability to increase cyclic AMP production in locust Malpighian tubules in vitro, has been isolated and characterized from whole heads of Locusta migratoria. The purified peptide stimulates fluid secretion by Malpighian tubules maximally in vitro. The primary structure of Locusta-DP was established as a 46 residue amidated peptide: MGMGPSLSIVNPMDVLRQRLLLEIARRRLRDAEEQIKANKDFLQQI-NH2. Locusta-DP has 48% sequence identity with Acheta-DP and 49% identity with Manduca-DH, and provides further evidence for the presence of a family of diuretic peptides in insects.

摘要

一种利尿肽，即飞蝗利尿肽（Locusta-DP），通过其在体外增加飞蝗马氏管中环磷酸腺苷（cAMP）生成的能力得以鉴定，现已从飞蝗（Locusta migratoria）的整个头部中分离并进行了特性分析。纯化后的该肽在体外能最大程度地刺激马氏管分泌液体。飞蝗利尿肽的一级结构确定为一种含46个残基的酰胺化肽：MGMGPSLSIVNPMDVLRQRLLLEIARRRLRDAEEQIKANKDFLQQI-NH2。飞蝗利尿肽与家蟋蟀利尿肽（Acheta-DP）具有48%的序列同一性，与烟草天蛾利尿激素（Manduca-DH）具有49%的同一性，这为昆虫中存在利尿肽家族提供了进一步的证据。