Koh Motohiro, Okamoto Eizo, Yamanaka Junichi, Fujimoto Jiro
Department of Surgery, Kyoritsu Hospital, Kawanishi, Japan.
Surg Today. 2006;36(5):457-64. doi: 10.1007/s00595-005-3183-4.
We studied the age-related changes in graft livers, and their impact on post-transplantation liver function and the growth of young recipient rats.
Rats aged 11-68 weeks old were studied as controls to assess liver histology, liver function, and body weight. We performed orthotopic liver transplantation using Kamada's cuff technique without arterial reconstruction. Young rats aged 11 weeks were randomized to receive livers from either 11-week-old donors (YD group) or 52-week-old donors (OD group). Recipient rats were killed 0, 8, or 16 weeks after surgery and we assessed the same variables as in the controls.
We confirmed an age-related increase in the average size of hepatocytes and their nuclei. These age-related changes persisted and progressed in the graft liver after transplantation. There were no significant differences in the levels of serum transaminases or total bilirubin between the YD and OD groups, but the serum albumin level was significantly lower in the OD group. The YD group grew normally, whereas the OD group recipients lagged significantly in gaining body weight.
We found that 52-week-old grafts transplanted into 11-week-old recipients resulted in deficient growth and a decline in serum albumin, suggesting that grafted old livers fail to produce enough protein to meet the demands of growth adequately.
我们研究了移植肝脏的年龄相关变化，以及它们对移植后肝功能和年轻受体大鼠生长的影响。
将11 - 68周龄的大鼠作为对照，评估肝脏组织学、肝功能和体重。我们采用Kamada袖套技术进行原位肝移植，不进行动脉重建。将11周龄的幼鼠随机分为两组，分别接受来自11周龄供体的肝脏（YD组）或52周龄供体的肝脏（OD组）。术后0、8或16周处死受体大鼠，评估与对照组相同的变量。
我们证实了肝细胞及其细胞核的平均大小与年龄相关的增加。这些与年龄相关的变化在移植后的移植肝脏中持续存在并进展。YD组和OD组之间血清转氨酶或总胆红素水平无显著差异，但OD组血清白蛋白水平显著较低。YD组正常生长，而OD组受体在体重增加方面明显滞后。
我们发现，将52周龄的移植物移植到11周龄的受体中会导致生长不足和血清白蛋白下降，这表明移植的老龄肝脏不能产生足够的蛋白质来充分满足生长需求。
