通过基质辅助激光解吸/电离飞行时间质谱法对巴西所罗门食鸟蛛毒腺进行肽谱分析。
Peptide profiling by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry of the Lasiodora parahybana tarantula venom gland.
作者信息
Guette Catherine, Legros Christian, Tournois Guewen, Goyffon Max, Célérier Marie-Louise
机构信息
USM 0505, Ecosystèmes et Interactions Toxiques, Muséum National d'Histoire Naturelle, 18 rue Buffon, 75231 Paris Cedex 05, France.
出版信息
Toxicon. 2006 May;47(6):640-9. doi: 10.1016/j.toxicon.2006.01.017. Epub 2006 Apr 25.
In order to establish a venom fingerprint and a peptide profile of the Lasiodora parahybana tarantula venom gland, we used conventional methods such as reversed phase liquid chromatography coupled to an electrospray-ionisation hybrid quadrupole time of flight mass spectrometer (LC/ESI-QqTOFMS), matrix-assisted laser desorption/ionization time-of-flight-MS (MALDI-TOFMS) and direct study of L. parahybana venom by nanospray-ionization QqTOFMS (nanoESI-QqTOFMS) and a new technology for the direct analysis of fresh tissues using MALDI-TOFMS. The analysis of the crude venom allowed the characterization of specific juvenile and adult biomarkers. In situ MALDI analysis of L. parahybana venom gland sections revealed different peptide expression levels all along the gland and non-processed peptide precursors, demonstrating the power of the method for the dynamic investigation of peptide evolution in the venom gland of spiders.
为了建立巴西所罗门食鸟蛛毒腺的毒液指纹图谱和肽谱，我们使用了常规方法，如反相液相色谱联用电喷雾电离混合四极杆飞行时间质谱仪（LC/ESI-QqTOFMS）、基质辅助激光解吸/电离飞行时间质谱仪（MALDI-TOFMS），以及通过纳米喷雾电离QqTOFMS（nanoESI-QqTOFMS）直接研究巴西所罗门食鸟蛛毒液，还有一种使用MALDI-TOFMS直接分析新鲜组织的新技术。对粗毒液的分析能够鉴定出特定的幼年和成年生物标志物。对巴西所罗门食鸟蛛毒腺切片进行原位MALDI分析，揭示了整个腺体不同的肽表达水平以及未加工的肽前体，证明了该方法在动态研究蜘蛛毒腺中肽进化方面的强大功能。
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