Escoubas P, Chamot-Rooke J, Stöcklin R, Whiteley B J, Corzo G, Genet R, Nakajima T
Suntory Institute for Bioorganic Research, Mishima-Gun, Shimamoto-Cho, Wakayamadai 1-1-1, Osaka 618, Japan.
Rapid Commun Mass Spectrom. 1999;13(18):1861-8. doi: 10.1002/(SICI)1097-0231(19990930)13:18<1861::AID-RCM730>3.0.CO;2-7.
The search for novel pharmacological tools in spider venoms involves the need for precise and reproducible species identification methods. As an addition to morphological analysis, we have developed venom fingerprinting by reversed-phase chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) as an efficient and precise venom identification tool. In order to compare the possible use of liquid chromatography electrospray ionization mass spectrometry (LC/ESI-MS) as an additional venom characterization tool, we have applied both methodologies to the study of several tarantula venom samples in the Pterinochilus murinus group. These species possess highly active venoms yet their taxonomy remains difficult. We demonstrate that both methodologies can be successfully applied to tarantula venom characterization. MALDI-TOFMS and ESI-MS gave similar overall profiles and allowed fine discrimination of samples. At least one venom sample was proven to belong to a completely different venom group. Coupling of ESI-MS with HPLC separation afforded a new dimension in venom analysis, with clear discrimination between components of similar Mr and gave a finer picture of venom composition, number of molecular species and molecular weight distribution.
在蜘蛛毒液中寻找新型药理工具需要精确且可重复的物种鉴定方法。作为形态学分析的补充，我们开发了通过反相色谱和基质辅助激光解吸/电离飞行时间质谱（MALDI-TOFMS）进行毒液指纹图谱分析，作为一种高效且精确的毒液鉴定工具。为了比较液相色谱电喷雾电离质谱（LC/ESI-MS）作为另一种毒液表征工具的可能用途，我们将这两种方法应用于对Pterinochilus murinus组中几种狼蛛毒液样本的研究。这些物种拥有高活性毒液，但其分类学仍然困难。我们证明这两种方法都可以成功应用于狼蛛毒液表征。MALDI-TOFMS和ESI-MS给出了相似的总体图谱，并能够对样本进行精细区分。至少有一个毒液样本被证明属于一个完全不同的毒液组。ESI-MS与HPLC分离相结合为毒液分析提供了一个新的维度，能够清晰区分相似分子量的成分，并更详细地呈现毒液组成、分子种类数量和分子量分布情况。
