Park Hue Jung, Lee Yoonki, Lee Jaemin, Park Chongmin, Moon Dong Eon
Department of Anesthesiology and Pain Medicine, School of Medicine, The Catholic University of Korea, Kangnam St. Mary's Hospital, 505 Banpo-Dong, Seocho-Gu, Seoul, Korea, 137-040.
Can J Anaesth. 2006 May;53(5):470-7. doi: 10.1007/BF03022619.
Botulinum toxin type A (BoNT-A) has been used to treat many disorders related to excessive muscle contraction, but there are few studies evaluating its effects on neuropathic pain. The aim of this study was to evaluate the analgesic effects of BoNT-A in a rat model of neuropathic pain.
Male Sprague-Dawley rats were prepared by ligating the left L5 and L6 spinal nerves to produce neuropathic pain. Seventy neuropathic rats were randomly assigned into seven groups. Either normal saline or BoNT-A (10, 20, 30 and 40 U.kg(-1)) was administered to the plantar surface of the affected left hind paw, and BoNT-A (30 and 40 U.kg(-1)) was administered into the unaffected right paw in order to determine the drug-induced systemic effect. Mechanical and cold allodynia were observed at pre-administration, one, three, five, seven and 15 days after drug administration, and were quantified by measuring withdrawal frequencies to stimuli with von Frey filament and 100% acetone, respectively. Rotarod performance was measured to detect drug-induced adverse motor effects.
The mean minimum withdrawal frequencies to mechanical and cold stimuli were 77 +/- 11 and 90 +/- 4.5%, 46 +/- 5 and 66 +/- 7%, 33 +/- 7 and 62 +/- 7%, 12 +/- 2.9 and 54 +/- 7.3% with 10, 20, 30 and 40 U.kg(-1) BoNT-A respectively (P < 0.05). Doses of 30 and 40 U.kg(-1) BoNT-A resulted in reduced rotarod performance time.
We conclude that peripherally administered BoNT-A reduces mechanical and cold allodynia in a rat model of neuropathic pain.
A型肉毒杆菌毒素（BoNT-A）已被用于治疗许多与肌肉过度收缩相关的疾病，但评估其对神经性疼痛影响的研究较少。本研究的目的是评估BoNT-A在神经性疼痛大鼠模型中的镇痛作用。
通过结扎雄性Sprague-Dawley大鼠的左L5和L6脊神经来制备神经性疼痛模型。将70只神经性大鼠随机分为七组。将生理盐水或BoNT-A（10、20、30和40 U.kg(-1)）注射到受影响的左后爪足底表面，并将BoNT-A（30和40 U.kg(-1)）注射到未受影响的右爪，以确定药物引起的全身效应。在给药前、给药后1天、3天、5天、7天和15天观察机械性和冷觉异常性疼痛，并分别通过测量对von Frey细丝和100%丙酮刺激的撤腿频率进行量化。测量转棒试验表现以检测药物引起的不良运动效应。
10、20、30和40 U.kg(-1) BoNT-A组对机械性和冷刺激的平均最小撤腿频率分别为77±11和90±4.5%、46±5和66±7%、33±7和62±7%、12±2.9和54±7.3%（P<0.05）。30和40 U.kg(-1) BoNT-A剂量导致转棒试验表现时间缩短。
我们得出结论，外周给予BoNT-A可减轻神经性疼痛大鼠模型中的机械性和冷觉异常性疼痛。
