吡立前列素（U-60,257B）和白三烯对大鼠子宫内膜基质细胞碱性磷酸酶活性的体外影响。

Effects of piriprost (U-60, 257B) and leukotrienes on alkaline phosphatase activity of rat endometrial stromal cells in vitro.

作者信息

Cejic S S, Kennedy T G

机构信息

Department of Physiology, University of Western Ontario, London, Canada.

出版信息

Prostaglandins. 1991 Aug;42(2):163-79. doi: 10.1016/0090-6980(91)90075-q.

DOI:10.1016/0090-6980(91)90075-q

PMID:1663640

Abstract

The present study investigated the effects of piriprost (U-60,257B; an inhibitor of LT synthesis) and various LTs on alkaline phosphatase (ALP) activity of rat endometrial stromal cells in vitro. Mature ovariectomized rats were pretreated with hormones to sensitize their uteri for the decidual cell reaction. Endometrial stromal cells were isolated and cultured for up to 72 hr with various treatments. The ALP activity in all experiments was significantly (p less than 0.01) higher at 72 hr than at 24 hr, irrespective of treatment. We examined the effects of 100 microM piriprost, with or without 1 microM LTB4, 0.01 microM LTC4, 0.1 microM LTD4 or 0.001 microM LTE4 on ALP activity. At 72 hr, as indicated by analyses of variance, there were significant interactions (p less than 0.01) between the effects of piriprost and the LTs. Piriprost by itself increased (p less than 0.01) ALP activity in all experiments, and a further increase (p less than 0.01) in ALP activity was observed when either LTB4, LTC4, LTD4 or LTE4 was added with piriprost. LTB4, LTD4, or LTE4 alone had inhibitory effects (p less than 0.01) while LTC4 alone had no effect. These studies suggest LTs may be involved in decidualization which, in vitro, is accompanied by an increase in endometrial ALP activity. However the exact role of LTs is still unclear.

摘要

本研究在体外研究了吡立前列素（U - 60,257B；白三烯合成抑制剂）和各种白三烯对大鼠子宫内膜基质细胞碱性磷酸酶（ALP）活性的影响。对成熟去卵巢大鼠进行激素预处理，使其子宫对蜕膜细胞反应敏感。分离子宫内膜基质细胞并进行各种处理，培养长达72小时。在所有实验中，无论处理如何，72小时时的ALP活性均显著高于24小时（p小于0.01）。我们研究了100 microM吡立前列素，分别联合或不联合1 microM白三烯B4、0.01 microM白三烯C4、0.1 microM白三烯D4或0.001 microM白三烯E4对ALP活性的影响。方差分析表明，在72小时时，吡立前列素和白三烯的作用之间存在显著的相互作用（p小于0.01）。在所有实验中，吡立前列素本身均可增加（p小于0.01）ALP活性，当白三烯B4、白三烯C4、白三烯D4或白三烯E4与吡立前列素联合使用时，可进一步增加（p小于0.01）ALP活性。单独使用白三烯B4、白三烯D4或白三烯E4具有抑制作用（p小于0.01），而单独使用白三烯C4则无作用。这些研究表明，白三烯可能参与蜕膜化过程，在体外，蜕膜化伴随着子宫内膜ALP活性的增加。然而，白三烯的确切作用仍不清楚。