Imaizumi Akira, Koseki Chie, Matsui Kazuhiko, Kojima Hiroyuki
Laboratories of Fermentation and Biotechnology, AJINOMOTO Co., Inc., Kanagawa, Japan.
Biosci Biotechnol Biochem. 2006 Apr;70(4):949-57. doi: 10.1271/bbb.70.949.
Using a DNA macroarray, we investigated the effects of rmf gene (encoding ribosome modulation factor) disruption on gene expression profiles in Escherichia coli. This strain showed a phosphate-starvation-like response in gene expression even under phosphate sufficient conditions; significant upregulation of the Pho regulon genes was observed. Further, the production of alkaline phosphatase, a product of the Pho regulon gene, phoA, increased in the rmf disruptant under a Pi sufficient condition. Furthermore, production of PhoC acid phosphatase/nucleoside pyrophosphate phosphotransferase derived from Morganella morganii also increased significantly in the rmf disruptant. We concluded that host modification by the rmf gene disruption has potential benefit in industrial enzyme production using Escherichia coli.
我们使用DNA宏阵列研究了rmf基因（编码核糖体调节因子）缺失对大肠杆菌基因表达谱的影响。即使在磷酸盐充足的条件下，该菌株在基因表达上也表现出类似磷酸盐饥饿的反应；观察到Pho调节子基因显著上调。此外，在磷酸盐充足的条件下，rmf缺失菌株中Pho调节子基因phoA的产物碱性磷酸酶的产量增加。此外，源自摩根氏摩根菌的PhoC酸性磷酸酶/核苷焦磷酸磷酸转移酶在rmf缺失菌株中的产量也显著增加。我们得出结论，rmf基因缺失引起的宿主修饰在利用大肠杆菌进行工业酶生产方面具有潜在益处。
