鉴定参与抑制人外周血淋巴细胞抗体产生的基因。

Identification of genes involved in the suppression of antibody production from human peripheral blood lymphocytes.

作者信息

Aiba Yoshihiro, Yamashita Makiko, Katakura Yoshinori, Furukawa Yuki, Matsumoto Shin-ei, Tomimatsu Kousuke, Teruya Kiichiro, Shirahata Sanetaka

机构信息

Graduate School of Systems Life Sciences, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.

出版信息

Biosci Biotechnol Biochem. 2006 Apr;70(4):966-70. doi: 10.1271/bbb.70.966.

DOI:10.1271/bbb.70.966

PMID:16636465

Abstract

Pretreatment with L-leucyl-L-leucine methyl ester (LLME) is a prerequisite for peripheral blood mononuclear cells (PBMCs) to produce antigen-specific antibodies when sensitized with an antigen. Little information, however, is available regarding the mechanisms involved in LLME-induced augmentation of antibody production from PBMCs that are antigen sensitized. In the present study, we attempted to identify the genes involved in the suppression of antibody production from PBMCs that was not treated with LLME, but sensitized with an antigen. Using subtractive screening, we obtained 63 independent genes, including 17 EST genes, that are specific for LLME-nontreated PBMC. Among these genes, the expression of heavy chain ferritin (H-ferritin), CC chemokine ligand 18 (CCL18), and matrix metalloproteinase 12 (MMP12) were augmented in LLME-nontreated PBMCs, suggesting that inflammatory factors might be involved in the suppression of antibody production in LLME-nontreated PBMCs.

摘要

用L-亮氨酰-L-亮氨酸甲酯（LLME）进行预处理是外周血单核细胞（PBMC）在用抗原致敏时产生抗原特异性抗体的前提条件。然而，关于LLME诱导抗原致敏的PBMC抗体产生增加所涉及的机制，目前所知甚少。在本研究中，我们试图鉴定那些参与抑制未用LLME处理但用抗原致敏的PBMC抗体产生的基因。通过消减筛选，我们获得了63个独立基因，包括17个EST基因，这些基因是未用LLME处理的PBMC所特有的。在这些基因中，重链铁蛋白（H-铁蛋白）、CC趋化因子配体18（CCL18）和基质金属蛋白酶12（MMP12）在未用LLME处理的PBMC中表达增加，这表明炎症因子可能参与了未用LLME处理的PBMC中抗体产生的抑制。