白细胞介素-10（IL-10）参与体外免疫外周血单个核细胞对抗体产生的抑制作用。
Involvement of IL-10 in the suppression of antibody production by in vitro immunized peripheral blood mononuclear cells.
机构信息
Graduate School of Systems Life Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan,
出版信息
Cytotechnology. 2007 Dec;55(2-3):71-7. doi: 10.1007/s10616-007-9088-x. Epub 2007 Aug 14.
Previously, we have established an in vitro immunization method to induce antigen-specific antibody-producing B cells. In the present study, we have attempted to clarify the mechanisms that regulate antibody production by in vitro immunized peripheral blood mononuclear cells (PBMC). Freshly isolated PBMC did not induce antibody production following in vitro immunization, but expressed the interleukin (IL)-10 gene. On the other hand, PBMC pretreated with L: -leucyl-L: -leucine methyl ester (LLME) induced antibody production, but did not express the IL-10 gene. IL-10 induced functional impairment of CD4(+) Th cells and CD11c(+) DC, resulting in the suppression of antibody production by in vitro immunized PBMC.
先前，我们已经建立了一种体外免疫方法来诱导抗原特异性产生抗体的 B 细胞。在本研究中，我们试图阐明调节体外免疫外周血单核细胞（PBMC）产生抗体的机制。新鲜分离的 PBMC 在体外免疫后不会诱导抗体产生，但表达白细胞介素（IL）-10 基因。另一方面，用 L：-亮氨酸-L：-亮氨酸甲酯（LLME）预处理的 PBMC 诱导抗体产生，但不表达 IL-10 基因。IL-10 诱导 CD4(+) Th 细胞和 CD11c(+) DC 的功能障碍，从而抑制体外免疫 PBMC 产生抗体。
Zotero 插件