Jinn Tzyy-Rong, Tu Wu-Chun, Lu Cha-I, Tzen Jason T C
Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, Taiwan 40227, Republic of China.
Appl Microbiol Biotechnol. 2006 Oct;72(6):1247-53. doi: 10.1007/s00253-006-0431-5. Epub 2006 Apr 25.
LqhIT(2), an insect specific neurotoxin from the venom of Leiurus quinquestriatus hebraeus, has been demonstrated to improve insecticidal efficacy of Autographa californica nuclear polyhedrosis virus (AcMNPV). A polyhedrin-positive recombinant AcMNPVvAcP(hsp70)EGFP/P(pag90)IT(2) was engineered for larvae to express the enhanced green fluorescence protein (EGFP) and LqhIT(2) under the control of P(hsp70) and P(pag90) promoters, respectively. This would allow a visual observation of the viral infection and an improvement of the insecticidal efficacy. The insecticidal activity of this recombinant baculovirus, a wild type AcMNPV and four other recombinant baculoviruses, was evaluated and compared in terms of mortality, body weight, median lethal time (LT(50)), and median lethal concentration (LC(50)). Insecticidal efficacy was unaltered when treated with vAcP(hsp70)EGFP, moderately improved when infected by vAcP(10)IT(2) (a P(10)-promoted LqhIT ( 2 ) gene), and significantly elevated when treated with vAcP(pag90)IT(2) or vAcP(hsp70)EGFP/P(pag90)IT(2). No apparent difference was observed in insecticidal efficacy when additional EGFP was expressed as a visible marker. These results suggest that recombinant AcMNPV vAcP(hsp70)EGFP/P(pag90)IT(2) may be used as an effective insecticide against Trichoplusia ni and other lepidopterous insect pests.
LqhIT(2)是一种来自以色列金蝎毒液的昆虫特异性神经毒素，已被证明可提高苜蓿银纹夜蛾核型多角体病毒（AcMNPV）的杀虫效果。构建了一种多角体蛋白阳性重组AcMNPVvAcP(hsp70)EGFP/P(pag90)IT(2)，使幼虫分别在P(hsp70)和P(pag90)启动子的控制下表达增强型绿色荧光蛋白（EGFP）和LqhIT(2)。这将有助于对病毒感染进行可视化观察，并提高杀虫效果。对这种重组杆状病毒、野生型AcMNPV和其他四种重组杆状病毒的杀虫活性进行了评估，并在死亡率、体重、半数致死时间（LT(50)）和半数致死浓度（LC(50)）方面进行了比较。用vAcP(hsp70)EGFP处理时杀虫效果未改变，用vAcP(10)IT(2)（一种P(10)启动的LqhIT(2)基因）感染时杀虫效果适度提高，用vAcP(pag90)IT(2)或vAcP(hsp70)EGFP/P(pag90)IT(2)处理时杀虫效果显著提高。当额外表达EGFP作为可见标记时，杀虫效果未观察到明显差异。这些结果表明，重组AcMNPV vAcP(hsp70)EGFP/P(pag90)IT(2)可作为一种有效的杀虫剂，用于防治粉纹夜蛾和其他鳞翅目害虫。
