Demir Volkan, Dincturk H Benan
Department of Molecular Biology and Genetics Faculty of Sciences and Letters, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey.
Mol Biol Rep. 2006 Mar;33(1):59-63. doi: 10.1007/s11033-005-5067-5.
Host cell physiology is known to play a crucial role in the expression of foreign genes in heterologous systems. Expression of archaeal genes in anaerobic or semi-anaerobic growth conditions of E. coli has been previously reported to be a means of improving solubility of some proteins. Here, we report that some of the Rosetta strains of E. coli, which harbour the rare tRNA genes for the expression of archaeal genes, favour semi-anaerobic conditions for the expression of putative FMN binding domain of glutamate synthase from Methanocaldococcus jannaschii at low inducer concentrations.
已知宿主细胞生理学在异源系统中外源基因的表达中起着关键作用。此前有报道称，在大肠杆菌的厌氧或半厌氧生长条件下表达古菌基因是提高某些蛋白质溶解度的一种方法。在此，我们报道，一些携带用于表达古菌基因的稀有tRNA基因的大肠杆菌Rosetta菌株，在低诱导剂浓度下，有利于在半厌氧条件下表达詹氏甲烷球菌谷氨酸合酶的假定FMN结合结构域。
