Belgacem Omar, Bowdler Andrew, Brookhouse Ian, Brancia Francesco L, Raptakis Emmanuel
Shimadzu Biotech, Wharfside, Trafford Wharf Road, Manchester M17 1GP, UK.
Rapid Commun Mass Spectrom. 2006;20(11):1653-60. doi: 10.1002/rcm.2486.
A commercial matrix-assisted laser desorption/ionisation time-of-flight (MALDI-ToF) instrument equipped with a curved field reflectron (CFR) was modified in order to perform collision-induced dissociation (CID) on a variety of biomolecules. The incorporation of a high-resolution ion gate together with a collision cell within the field-free region allowed tandem mass analysis (MS/MS), without the necessity to decelerate the precursor ions prior to activation. The simultaneous detection of all product ions remained possible by using the CFR. To test the MS/MS performances, ACTH (fragment 1-17), a complex high mannose carbohydrate (Man)(8)(GlcNac)(2) and a lysophosphatidylcholine lipid (18:1) were analysed on the modified instrument. Direct comparison with the low-energy product ion spectra, acquired on a MALDI quadrupole ion trap (QIT) two-stage reflectron time-of flight (ReToF) mass spectrometer, showed significant differences in the types of product ions observed. The additional ions detected were a clear indication of the high-energy fragmentation processes occurring in the collision cell.
配备了弯曲场反射器（CFR）的商用基质辅助激光解吸/电离飞行时间（MALDI-ToF）仪器经过改装，以便对多种生物分子进行碰撞诱导解离（CID）。在无场区引入高分辨率离子门和碰撞池，实现了串联质谱分析（MS/MS），无需在激活前对前体离子进行减速。通过使用CFR，仍然可以同时检测所有产物离子。为了测试MS/MS性能，在改装后的仪器上分析了促肾上腺皮质激素（ACTH，片段1-17）、一种复杂的高甘露糖碳水化合物（Man）(8)(GlcNac)(2)和一种溶血磷脂酰胆碱脂质（18:1）。与在MALDI四极杆离子阱（QIT）两级反射器飞行时间（ReToF）质谱仪上获得的低能量产物离子光谱进行直接比较，结果表明观察到的产物离子类型存在显著差异。检测到的额外离子清楚地表明碰撞池中发生了高能碎片化过程。
