Devakumar Arugadoss, Thompson Matthew S, Reilly James P
Department of Chemistry, Indiana University, Bloomington, IN 47405, USA.
Rapid Commun Mass Spectrom. 2005;19(16):2313-20. doi: 10.1002/rcm.2058.
The 157 nm photofragmentation of native and derivatized oligosaccharides was studied in a linear ion trap and in a home-built matrix-assisted laser desorption/ionization (MALDI) tandem time-of-flight (TOF/TOF) mass spectrometer, and the results were compared with collision-induced dissociation (CID) experiments. Photodissociation produces product ions corresponding to high-energy fragmentation pathways; for cation-derivatized oligosaccharides, it yields strong cross-ring fragment ions and provides better sequence coverage than low- and high-energy CID experiments. On the other hand, for native oligosaccharides, CID yielded somewhat better sequence coverage than photodissociation. The ion trap enables CID hybrid MS3 experiments on the high-energy fragment ions obtained from photodissociation.
在线性离子阱和自行搭建的基质辅助激光解吸/电离（MALDI）串联飞行时间（TOF/TOF）质谱仪中研究了天然和衍生化寡糖的157 nm光碎裂，并将结果与碰撞诱导解离（CID）实验进行了比较。光解离产生对应于高能碎裂途径的产物离子；对于阳离子衍生化寡糖，它产生强的跨环碎片离子，并且比低能和高能CID实验提供更好的序列覆盖。另一方面，对于天然寡糖，CID产生的序列覆盖比光解离略好。离子阱能够对从光解离获得的高能碎片离子进行CID混合MS3实验。
